HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

9p21 deletion in the diagnosis of malignant mesothelioma, using fluorescence in situ hybridization analysis.

Abstract
Homozygous deletion of 9p21, the locus harboring the p16 gene, has been reported as one of the most common genetic alterations in malignant mesotheliomas (MMs). Previous studies showed that this alteration might be useful for differentiating benign from malignant mesothelial tumors in cytology and surgical specimens. Although the diagnostic utility of 9p21 homozygous deletion by fluorescence in situ hybridization (FISH) analysis has been reported only recently, it has not been well demonstrated. The purpose of this study is to evaluate the diagnostic utility of 9p21 homozygous deletion assessed by FISH in mesothelial neoplasm and hyperplasia of Japanese patients using paraffin-embedded tissue. Simultaneously, p16 protein immunoexpression was explored as a potential diagnostic aid. FISH analysis demonstrated 9p21 deletion in 35 of 40 cases with MM (88%) (P < 0.001). In contrast, no cases of adenomatoid tumor, benign mesothelial multicystic tumor, reactive mesothelial hyperplasia or pleuritis showed 9p21 deletion (P < 0.005). 9p21 homozygous deletion correlated well with p16 protein expression in the tumor cells. Our study suggests that 9p21 homozygous deletion assessed by FISH on paraffin-embedded tissue may be very useful for differentiating MM from reactive mesothelial proliferation.
AuthorsMaiko Takeda, Takahiko Kasai, Yasunori Enomoto, Masato Takano, Kouhei Morita, Eiji Kadota, Akitaka Nonomura
JournalPathology international (Pathol Int) Vol. 60 Issue 5 Pg. 395-9 (May 2010) ISSN: 1440-1827 [Electronic] Australia
PMID20518890 (Publication Type: Journal Article)
Chemical References
  • Biomarkers, Tumor
  • DNA, Neoplasm
Topics
  • Biomarkers, Tumor (genetics, metabolism)
  • Chromosomes, Human, Pair 9
  • DNA, Neoplasm (analysis)
  • Epithelium (pathology)
  • Female
  • Gene Deletion
  • Gene Dosage
  • Genes, p16
  • Heart Neoplasms (diagnosis, genetics, metabolism)
  • Humans
  • In Situ Hybridization, Fluorescence (methods)
  • Mesothelioma (diagnosis, genetics, metabolism)
  • Pericardium (metabolism, pathology)
  • Peritoneal Neoplasms (diagnosis, genetics, metabolism)
  • Pleural Neoplasms (diagnosis, genetics, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: