Abstract |
Glioblastoma cells with stem-like properties control brain tumour growth and recurrence. Here, we show that endogenous neural precursor cells perform an anti-tumour response by specifically targeting stem-like brain tumour cells. In vitro, neural precursor cells predominantly express bone morphogenetic protein-7; bone morphogenetic protein-7 is constitutively released from neurospheres and induces canonical bone morphogenetic protein signalling in stem-like glioblastoma cells. Exposure of human and murine stem-like brain tumour cells to neurosphere-derived bone morphogenetic protein-7 induces tumour stem cell differentiation, attenuates stem-like marker expression and reduces self-renewal and the ability for tumour initiation. Neurosphere-derived or recombinant bone morphogenetic protein-7 reduces glioblastoma expansion from stem-like cells by down-regulating the transcription factor Olig2. In vivo, large numbers of bone morphogenetic protein-7-expressing neural precursors encircle brain tumours in young mice, induce canonical bone morphogenetic protein signalling in stem-like glioblastoma cells and can thereby attenuate tumour formation. This anti-tumour response is strongly reduced in older mice. Our results indicate that endogenous neural precursor cells protect the young brain from glioblastoma by releasing bone morphogenetic protein-7, which acts as a paracrine tumour suppressor that represses proliferation, self-renewal and tumour-initiation of stem-like glioblastoma cells.
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Authors | Sridhar Reddy Chirasani, Alexander Sternjak, Peter Wend, Stefan Momma, Benito Campos, Ilaria M Herrmann, Daniel Graf, Thimios Mitsiadis, Christel Herold-Mende, Daniel Besser, Michael Synowitz, Helmut Kettenmann, Rainer Glass |
Journal | Brain : a journal of neurology
(Brain)
Vol. 133
Issue Pt 7
Pg. 1961-72
(Jul 2010)
ISSN: 1460-2156 [Electronic] England |
PMID | 20513660
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Bone Morphogenetic Protein 7
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Topics |
- Animals
- Bone Morphogenetic Protein 7
(metabolism)
- Brain Neoplasms
(metabolism, pathology, prevention & control)
- Glioblastoma
(metabolism, pathology, prevention & control)
- Humans
- Mice
- Mice, Inbred C57BL
- Mice, Inbred NOD
- Mice, SCID
- Neoplastic Stem Cells
(metabolism, pathology)
- Neurons
(metabolism, pathology)
- Stem Cells
(metabolism, pathology)
- Tumor Cells, Cultured
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