Trypanosoma cruzi: modulation of HSP70 mRNA stability by untranslated regions during heat shock.

Abstract

Gene regulation in trypanosomatids occurs mainly by post-transcriptional mechanisms modulating mRNA stability and translation. We have investigated heat shock protein (HSP) 70 gene regulation in Trypanosoma cruzi, the causal agent of Chagas' disease. The HSP70 mRNA's half-life increases after heat shock, and the stabilization is dependent on protein synthesis. In a cell-free RNA decay assay, a U-rich region in the 3' untranslated region (UTR) is a target for degradation, which is reduced when in the presence of protein extracts from heat shocked cells. In a transfected reporter gene assay, both the 5'- and 3'-UTRs confer temperature-dependent regulation. Both UTRs must be present to increase mRNA stability at 37 degrees C, indicating that the 5'- and 3'-UTRs act cooperatively to stabilize HSP70 mRNA during heat shock. We conclude that HSP70 5'- and 3'-UTRs regulate mRNA stability during heat shock in T. cruzi.

• Authors: Deivid C Rodrigues, Rosane Silva, Edson Rondinelli, Turán P Urményi
• Journal: Experimental parasitology (Exp Parasitol) Vol. 126 Issue 2 Pg. 245-53 (Oct 2010) ISSN: 1090-2449 [Electronic] United States
• PMID: 20493845 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2010 Elsevier Inc. All rights reserved.

Chemical References

• 3' Untranslated Regions
• 5' Untranslated Regions
• DNA, Protozoan
• HSP70 Heat-Shock Proteins
• RNA, Messenger
• Chloramphenicol O-Acetyltransferase

Topics

• 3' Untranslated Regions (genetics, physiology)
• 5' Untranslated Regions (genetics, physiology)
• Base Sequence
• Blotting, Northern
• Chloramphenicol O-Acetyltransferase (genetics, metabolism)
• DNA, Protozoan (chemistry, genetics, metabolism)
• Gene Expression Regulation (physiology)
• HSP70 Heat-Shock Proteins (genetics, metabolism)
• Half-Life
• Hot Temperature
• Plasmids (genetics, metabolism)
• RNA, Messenger (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Transfection
• Trypanosoma cruzi (genetics, metabolism)