The selective role of myosin VI in lymphoid leukemia cell migration.

Abstract

Several myosin isotypes are discussed to be involved in the migration of various cells ranging from tumor cells to leukocytes. We investigated the involvement of myosins II and VI in the lymphoid leukemia cells lines Jurkat, NB4, Dohh-2, and Molt-4 by a three-dimensional, collagen-based migration assay. Down-regulation of myosin VI by siRNA significantly reduced the migratory activity of all cells, whereas the pharmacological inhibition of non-muscle myosin II using blebbistatin had only marginal influence. Therefore, in contrast to differentiated leukocytes and cells from solid tumors, myosin VI plays a crucial role in the migration of leukemic cells.

• Authors: Jbireal M Ali Jbireal, Carina Strell, Bernd Niggemann, Kurt Zänker, Frank Entschladen
• Journal: Leukemia research (Leuk Res) Vol. 34 Issue 12 Pg. 1656-62 (Dec 2010) ISSN: 1873-5835 [Electronic] England
• PMID: 20493527 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010 Elsevier Ltd. All rights reserved.

Chemical References

• Heterocyclic Compounds, 4 or More Rings
• Neoplasm Proteins
• RNA, Small Interfering
• myosin VI
• blebbistatin
• Myosin Type II
• Myosin Heavy Chains

Topics

• Cell Movement
• Heterocyclic Compounds, 4 or More Rings (pharmacology)
• Humans
• Jurkat Cells
• K562 Cells
• Leukemia, Lymphoid (genetics, metabolism, pathology)
• Myosin Heavy Chains (genetics, metabolism)
• Myosin Type II (antagonists & inhibitors, genetics, metabolism)
• Neoplasm Proteins (genetics, metabolism)
• RNA, Small Interfering (genetics)