Abstract | OBJECTIVES: The aim of this study was to evaluate and compare concentration of selected human beta-defensins (hBD-1, hBD-2) in cervico-vaginal lavage (CVL), obtained from women with candidiasis, chlamydiasis and other bacterial infections. MATERIAL AND METHODS:
beta-defensins were detected quantitatively by RT-PCR (7000 Taqman, Applied Biosystems) in cervico-vaginal lavage collected from 120 (79 women in the study group and 41 controls) non-pregnant women, aged 18-40 (mean age 28.5 +/- 6.29). The study group patients were divided into three subgroups on the basis of clinical and microbiological diagnosis: women with candidiasis (n=13); with chlamydiasis (n=13), and with other bacterial infections (n=12). RESULTS: The highest count of hBD-1 RNA copies was found in women with bacterial infections and candidiasis (335.84 and 320.10 respectively), and hBD-2--with chlamydiasis. The difference between RNA copies of hBD-1/microg in candidiasis, chlamydiasis and bacterial pathogens was statistically significant; for hBD-2 only in case of chlamydiasis. CONCLUSIONS: Chlamydia trachomatis infection activates the production of hBD-2. Candida albicans, Chlamydia trachomatis, and bacterial pathogens induced variable increases of hBD-1 concentration.
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Authors | Barbara Wiechuła, Krzysztof Cholewa, Alicja Ekiel, Małgorzata Romanik, Hanna Dolezych, Gayane Martirosian |
Journal | Ginekologia polska
(Ginekol Pol)
Vol. 81
Issue 4
Pg. 268-71
(Apr 2010)
ISSN: 0017-0011 [Print] Poland |
Vernacular Title | Ekspresja hBD-1 i hBD-2 w popłuczynach pochwowo-szyjkowych z dróg rodnych kobiet w stanach zapalnych wywołanych drobnoustrojami. |
PMID | 20476598
(Publication Type: Comparative Study, English Abstract, Journal Article)
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Chemical References |
- Biomarkers
- DEFB1 protein, human
- DEFB4A protein, human
- beta-Defensins
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Topics |
- Adult
- Biomarkers
(metabolism)
- Candidiasis, Vulvovaginal
(diagnosis, metabolism)
- Cervix Mucus
(microbiology)
- Chlamydia Infections
(diagnosis, metabolism)
- Colony Count, Microbial
- Female
- Genital Diseases, Female
(diagnosis, metabolism, microbiology)
- Humans
- Reverse Transcriptase Polymerase Chain Reaction
- Sexually Transmitted Diseases, Bacterial
(diagnosis, metabolism)
- Vagina
(metabolism)
- Vaginal Smears
(methods)
- Young Adult
- beta-Defensins
(metabolism)
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