Multimodal nonlinear optical imaging of atherosclerotic plaque development in myocardial infarction-prone rabbits.

Abstract

Label-free imaging of bulk arterial tissue is demonstrated using a multimodal nonlinear optical microscope based on a photonic crystal fiber and a single femtosecond oscillator operating at 800 nm. Colocalized imaging of extracellular elastin fibers, fibrillar collagen, and lipid-rich structures within aortic tissue obtained from atherosclerosis-prone myocardial infarction-prone Watanabe heritable hyperlipidemic (WHHLMI) rabbits is demonstrated through two-photon excited fluorescence, second harmonic generation, and coherent anti-Stokes Raman scattering, respectively. These images are shown to differentiate healthy arterial wall, early atherosclerotic lesions, and advanced plaques. Clear pathological changes are observed in the extracellular matrix of the arterial wall and correlated with progression of atherosclerotic disease as represented by the age of the WHHLMI rabbits.

• Authors: Alex C T Ko, Andrew Ridsdale, Michael S D Smith, Leila B Mostaço-Guidolin, Mark D Hewko, Adrian F Pegoraro, Elicia K Kohlenberg, Bernie Schattka, Masashi Shiomi, Albert Stolow, Michael G Sowa
• Journal: Journal of biomedical optics (J Biomed Opt) 2010 Mar-Apr Vol. 15 Issue 2 Pg. 020501 ISSN: 1560-2281 [Electronic] United States
• PMID: 20459215 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Animals
• Coronary Artery Disease (complications, pathology)
• Equipment Design
• Equipment Failure Analysis
• Microscopy, Confocal (instrumentation)
• Myocardial Infarction (etiology, pathology)
• Nonlinear Dynamics
• Rabbits
• Reproducibility of Results
• Sensitivity and Specificity