Abstract | BACKGROUND: Reports of ischemia-reperfusion (I/R) injury in vivo describe experiments in which lesions are induced by the physical procedure of clamping, (I/RIC). We compare this procedure with a pharmacologic technique in which I/R injury is induced by drug superfusion (I/RID). MATERIALS AND METHODS: RESULTS: In both ischemia models, we observed significant differences in Evans blue (vascular permeability) and LDH (tissue injury) concentrations during the reperfusion period compared with the control group. I/RIC always induced greater injury. However, proportionally, the degree of protection was similar in the two models for the different treatments assayed. This indicates that the pathophysiologic mechanisms are the same. CONCLUSIONS: Our I/RID model induces a significant intestinal alteration during the reperfusion period and, also in general terms, this alteration is prevented or worsened in a similar and proportional way to that observed when using the classic I/RIC model. The I/RID model helps to explain the development and evolution of pathologies characterized by the induction of intermittent vasospasms that produce transitory reductions in vascular perfusion, which in turn can generate ROS though an I/R mechanism.
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Authors | J Giménez-Crouseilles, P Puig-Parellada |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 168
Issue 1
Pg. 34-41
(Jun 01 2011)
ISSN: 1095-8673 [Electronic] United States |
PMID | 20452622
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier Inc. All rights reserved. |
Chemical References |
- Reactive Oxygen Species
- Nitric Oxide
- Allopurinol
- Arginine
- Catalase
- Superoxide Dismutase
- NG-Nitroarginine Methyl Ester
|
Topics |
- Allopurinol
(adverse effects)
- Animals
- Arginine
(adverse effects)
- Catalase
(adverse effects)
- Constriction
- Intestine, Small
(blood supply, metabolism, pathology)
- Male
- Models, Animal
- NG-Nitroarginine Methyl Ester
(adverse effects)
- Nitric Oxide
(metabolism)
- Rats
- Rats, Wistar
- Reactive Oxygen Species
(metabolism)
- Reperfusion Injury
(chemically induced, metabolism, pathology)
- Superoxide Dismutase
(adverse effects)
- Time Factors
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