Clinical evaluation of developed PCR-based method with hydrolysis probes for TOP2A copy number evaluation in breast cancer samples.

Abstract

OBJECTIVES:

The objective of this study was to develop a new real time PCR-based method for quantitative detection of topoisomerase II alpha (TOP2A) aberrations and to evaluate its clinical utility in breast cancer.

DESIGN AND METHODS:

The method applied dually labelled hydrolysis probes and Pfaffl quantification method. The study group consisted of 83 consecutive breast cancer patients.

RESULTS:

In the examined tumour samples median TOP2A gene dosage was 1.08 (range 0.34-7.55). TOP2A amplifications were found in 12 tumours (14.5%), no deletion was detected. Statistically significant positive correlation of TOP2A gene dosage with nodal status, tumour grade, and HER2 protein status was found. TOP2A status also correlated with disease free survival.

CONCLUSIONS:

The newly developed real time PCR assay showed to be fast and easy to perform. Determined by the method TOP2A gene dosage was shown to be a potent prognostic factor in breast cancer.

• Authors: Anna Zaczek, Aleksandra Markiewicz, Janusz Jaśkiewicz, Tadeusz Pieńkowski, Piotr Rhone, Jacek Jassem, Marzena Wełnicka-Jaśkiewicz
• Journal: Clinical biochemistry (Clin Biochem) Vol. 43 Issue 10-11 Pg. 891-8 (Jul 2010) ISSN: 1873-2933 [Electronic] United States
• PMID: 20441774 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

Chemical References

• Antigens, Neoplasm
• DNA-Binding Proteins
• Oligonucleotide Probes
• Poly-ADP-Ribose Binding Proteins
• DNA Topoisomerases, Type II
• TOP2A protein, human

Topics

• Adult
• Aged
• Aged, 80 and over
• Antigens, Neoplasm (genetics)
• Breast Neoplasms (genetics)
• DNA Topoisomerases, Type II (genetics)
• DNA-Binding Proteins (genetics)
• Female
• Gene Dosage (genetics)
• Humans
• Hydrolysis
• Middle Aged
• Oligonucleotide Probes (genetics)
• Poly-ADP-Ribose Binding Proteins
• Reverse Transcriptase Polymerase Chain Reaction (methods)