Nitric oxide mechanism in the protective effect of naringin against post-stroke depression (PSD) in mice.

Abstract

AIM:

The present study has been designed to explore the nitric oxide mechanism in the protective effect of naringin against I/R induced neurobehavioral alterations, oxidative damage and mitochondrial dysfunction in mice.

MAIN METHODS:

Laca mice (25-30 g) were subjected twice to BCCAO occlusion (5 min) at the interval of 10 min, followed by 96 h reperfusion. Naringin (50 and 100 mg/kg) was administered for 10 days, starting 7 days before the animals were subjected to I/R injury. On day 10, various neurobehavioral parameters followed by biochemical parameters and mitochondrial enzyme complex activities were assessed.

KEY FINDINGS:

Ischemia reperfusion injury caused significant (increased immobility period, neurological score and decreased locomotor activity) oxidative damage (increased lipid peroxidation and nitrite concentration and depleted reduced glutathione, glutathione-S-transferase, superoxide dismutase and catalase) and altered mitochondrial enzyme complex activities (complex I to IV) as compared to sham treatment. Naringin (50 and 100 mg/kg) treatment significantly attenuated neurobehavioral alterations, oxidative damage and restored mitochondrial enzyme complex activities as compared to control (ischemia reperfusion) group. Further, protective effect of naringin (50 mg/kg) was attenuated by l-arginine (100 mg/kg) or sildenafil (5 mg/kg) pretreatment. Further, L-NAME (10 mg/kg) or 7-NI (10 mg/kg) pretreatment with naringin (50 mg/kg) significantly potentiated their protective effect as compared to their treatment alone.

SIGNIFICANCE:

The present study suggests the involvement of nitric oxide mechanism in the protective effect of naringin against post-stroke depression induced neurobehavioral, biochemical and cellular alterations in mice.

• Authors: Aditi Aggarwal, Vaibhav Gaur, Anil Kumar
• Journal: Life sciences (Life Sci) Vol. 86 Issue 25-26 Pg. 928-35 (Jun 19 2010) ISSN: 1879-0631 [Electronic] Netherlands
• PMID: 20433854 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2010 Elsevier Inc. All rights reserved.

Chemical References

• Flavanones
• Protective Agents
• Nitric Oxide
• naringin

Topics

• Animals
• Cell Respiration (drug effects)
• Depression (drug therapy, etiology, prevention & control)
• Flavanones (pharmacology, therapeutic use)
• Mice
• Motor Activity (drug effects)
• Nitric Oxide (metabolism)
• Oxidation-Reduction (drug effects)
• Oxidative Stress (drug effects)
• Protective Agents (pharmacology, therapeutic use)
• Rats
• Reperfusion Injury (drug therapy, enzymology)
• Stroke (complications)
• Swimming