Transmission and scanning electron microscopic observations of mouse adrenocortical adenoma cells (Y-1) in non-stimulated and stimulated states.

Abstract

ACTH sensitive murine adrenocortical adenoma cells (Y-1) were examined by transmission and scanning electron microscopes under non-stimulated and stimulated conditions. They showed the ultrastructural characteristics which were fairly different from those of the original adrenocortical cells of LAF1 mice. The smooth-surfaced endoplasmic reticulum (sER) were poorly developed, and the mitochondrial cristae showed lamellar rather than tubular or tubulo-vesicular type. Administration of ACTH induced rounding-up of the Y-1 cells with slight increase in sER, BUt no mitochondria with vesicular inner structure appeared. The effects of dbcAMP on the morphology of the Y-1 cells were similar to those of ACTH except that the rounding-up occurred more rapidly and uniformly.

• Authors: A Kawaoi, T Uchida, T Okano
• Journal: Acta pathologica japonica (Acta Pathol Jpn) Vol. 27 Issue 6 Pg. 841-56 (Nov 1977) ISSN: 0001-6632 [Print] Australia
• PMID: 204154 (Publication Type: Journal Article)

Chemical References

• Bucladesine
• Adrenocorticotropic Hormone

Topics

• Adenoma (metabolism, ultrastructure)
• Adrenal Cortex (ultrastructure)
• Adrenal Cortex Neoplasms (metabolism, ultrastructure)
• Adrenal Gland Neoplasms (ultrastructure)
• Adrenocorticotropic Hormone (pharmacology)
• Bucladesine (pharmacology)
• Cell Line
• Cytoplasmic Granules (ultrastructure)
• Microscopy, Electron, Scanning