Establishment of a resource population of SLA haplotype-defined Korean native pigs.

Abstract

The highly polymorphic porcine major histocompatibility complex (MHC), or the swine leukocyte antigens (SLA), has been repeatedly associated with variations in swine immune response to pathogens and vaccines as well as with production traits. The SLA antigens are also important targets for immunological recognition of foreign tissue grafts. We recently established a resource population of Korean native pigs as models for human transplantation and xenotransplantation research. In this study, 115 animals derived from three generations of the Korean native pigs were genotyped for three SLA class I (SLA-2, SLA-3 and SLA-1) and three SLA class II loci (DRB1, DQB1, DQA) using PCR with sequence-specific primers (PCR-SSP) at the allele group resolution. A total of seven SLA haplotypes (Lr-5.34, Lr-7.23, Lr-31.13, Lr-56.23, Lr-56.30, Lr-59.1, Lr-65.34), comprising six unique class I and five unique class II haplotypes, were characterized in the founding animals. Class I haplotype Lr-65.0 and class II haplotype Lr-0.34 were novel; and together with Lr-56.0 these haplotypes appeared to be breed-specific. In the progeny population, Lr-7.23 and Lr-56.30 appeared to be the most prevalent haplotypes with frequencies of 34.7% and 31.6%, respectively; the overall homozygosity was 27.4%. This resource population of SLA-defined Korean native pigs will be useful as large animal models for various transplantation and xenotransplantation experiments, as well as for dissecting the roles of SLA proteins in swine disease resistance and production traits.

• Authors: Han-Ok Cho, Chak-Sum Ho, Yu-Joo Lee, In-Cheol Cho, Sung-Soo Lee, Moon-Suck Ko, Chankyu Park, Douglas M Smith, Jin-Tae Jeon, Jun-Heon Lee
• Journal: Molecules and cells (Mol Cells) Vol. 29 Issue 5 Pg. 493-9 (May 2010) ISSN: 0219-1032 [Electronic] Korea (South)
• PMID: 20396963 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Histocompatibility Antigens Class I
• Histocompatibility Antigens Class II
• swine leukocyte antigen
• DNA

Topics

• Animals
• DNA (analysis)
• Gene Frequency
• Genotype
• Haplotypes
• Health Resources
• Histocompatibility Antigens Class I
• Histocompatibility Antigens Class II (genetics)
• Homozygote
• Humans
• Immunogenetics
• Models, Animal
• Organ Transplantation
• Species Specificity
• Swine (genetics, immunology)