Abstract |
A safe and effective adjuvant is important to develop vaccines against highly pathogenic avian influenza virus. Chitosan, a derivative from the natural amino polysaccharide chitin, has been proved to be an effective adjuvant for inactivated influenza virus vaccine. In this study, protective immunity in mice provided by chitosan-adjuvanted inactivated H5N1 vaccine was compared with that from an aluminum hydroxide-adjuvanted one. Mice were injected intraperitoneally once or twice with various dosages of inactivated vaccine alone or in combination with an adjuvant ( chitosan or aluminum hydroxide). To test the immunization effect, mice were challenged with a lethal dose of H5N1 virus. The results showed that the adjuvanted vaccines were more effective than adjuvant-free ones in inducing humoral immune responses and protecting mice against lethal challenge. Chitosan was comparable to the alum adjuvant in efficacy. These findings indicated that chitosan might be a candidate adjuvant for parenteral administration of inactivated influenza vaccines.
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Authors | Haiyan Chang, Xiaoman Li, Yong Teng, Yan Liang, Bo Peng, Fang Fang, Ze Chen |
Journal | DNA and cell biology
(DNA Cell Biol)
Vol. 29
Issue 9
Pg. 563-8
(Sep 2010)
ISSN: 1557-7430 [Electronic] United States |
PMID | 20380570
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adjuvants, Immunologic
- Influenza Vaccines
- Vaccines, Inactivated
- Aluminum Hydroxide
- Chitosan
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Topics |
- Adjuvants, Immunologic
(pharmacology)
- Aluminum Hydroxide
(immunology)
- Animals
- Antibody Formation
(immunology)
- Body Weight
(immunology)
- Chitosan
(immunology)
- Female
- Influenza A Virus, H5N1 Subtype
(immunology, physiology)
- Influenza Vaccines
(administration & dosage, immunology)
- Injections, Intraperitoneal
- Lung
(virology)
- Mice
- Mice, Inbred BALB C
- Survival Rate
- Vaccines, Inactivated
(administration & dosage, immunology)
- Viral Load
(immunology)
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