Abstract |
The conserved fission yeast protein Rad26(ATRIP) preserves genomic stability by occupying central positions within DNA-structure checkpoint pathways. It is also required for proper cellular morphology, chromosome stability and following treatment with microtubule poisons. Here, we report that mutation of a putative nuclear export sequence in Rad26(ATRIP) disrupted its cytoplasmic localization in untreated cells and conferred abnormal cellular morphology, minichromosome instability and sensitivity to microtubule poisons without affecting DNA-structure checkpoint signaling. This mutation also disrupted a delay to spindle-pole-body separation that occurred following microtubule damage in G(2). Together, these results demonstrate that Rad26(ATRIP) participates in two genetically defined checkpoint pathways--one that responds to genomic damage and the other to microtubule damage. This response to microtubule damage delays spindle-pole-body separation and, in doing so, might preserve both cellular morphology and chromosome stability.
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Authors | Matthew Herring, Nick Davenport, Kendra Stephan, Shawna Campbell, Rebecca White, Jonathan Kark, Tom D Wolkow |
Journal | Journal of cell science
(J Cell Sci)
Vol. 123
Issue Pt 9
Pg. 1537-45
(May 01 2010)
ISSN: 1477-9137 [Electronic] England |
PMID | 20375067
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Benzimidazoles
- Carbamates
- Cell Cycle Proteins
- DNA, Fungal
- Mad2 Proteins
- Nuclear Export Signals
- Nuclear Proteins
- Recombinant Fusion Proteins
- Schizosaccharomyces pombe Proteins
- mad2 protein, S pombe
- rad26 protein, S pombe
- carbendazim
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Topics |
- Alleles
- Amino Acid Sequence
- Benzimidazoles
(pharmacology)
- Carbamates
(pharmacology)
- Cell Cycle Proteins
(chemistry, metabolism)
- Chromosomal Instability
(drug effects)
- DNA Damage
- DNA, Fungal
(metabolism)
- G2 Phase
(drug effects)
- Interphase
(drug effects)
- Mad2 Proteins
- Microtubules
(metabolism)
- Mitosis
(drug effects)
- Molecular Sequence Data
- Nuclear Export Signals
- Nuclear Proteins
(metabolism)
- Recombinant Fusion Proteins
(metabolism)
- Schizosaccharomyces
(cytology, drug effects, metabolism)
- Schizosaccharomyces pombe Proteins
(chemistry, metabolism)
- Signal Transduction
(drug effects)
- Spindle Apparatus
(drug effects, metabolism)
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