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Fission yeast Rad26ATRIP delays spindle-pole-body separation following interphase microtubule damage.

Abstract
The conserved fission yeast protein Rad26(ATRIP) preserves genomic stability by occupying central positions within DNA-structure checkpoint pathways. It is also required for proper cellular morphology, chromosome stability and following treatment with microtubule poisons. Here, we report that mutation of a putative nuclear export sequence in Rad26(ATRIP) disrupted its cytoplasmic localization in untreated cells and conferred abnormal cellular morphology, minichromosome instability and sensitivity to microtubule poisons without affecting DNA-structure checkpoint signaling. This mutation also disrupted a delay to spindle-pole-body separation that occurred following microtubule damage in G(2). Together, these results demonstrate that Rad26(ATRIP) participates in two genetically defined checkpoint pathways--one that responds to genomic damage and the other to microtubule damage. This response to microtubule damage delays spindle-pole-body separation and, in doing so, might preserve both cellular morphology and chromosome stability.
AuthorsMatthew Herring, Nick Davenport, Kendra Stephan, Shawna Campbell, Rebecca White, Jonathan Kark, Tom D Wolkow
JournalJournal of cell science (J Cell Sci) Vol. 123 Issue Pt 9 Pg. 1537-45 (May 01 2010) ISSN: 1477-9137 [Electronic] England
PMID20375067 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
Chemical References
  • Benzimidazoles
  • Carbamates
  • Cell Cycle Proteins
  • DNA, Fungal
  • Mad2 Proteins
  • Nuclear Export Signals
  • Nuclear Proteins
  • Recombinant Fusion Proteins
  • Schizosaccharomyces pombe Proteins
  • mad2 protein, S pombe
  • rad26 protein, S pombe
  • carbendazim
Topics
  • Alleles
  • Amino Acid Sequence
  • Benzimidazoles (pharmacology)
  • Carbamates (pharmacology)
  • Cell Cycle Proteins (chemistry, metabolism)
  • Chromosomal Instability (drug effects)
  • DNA Damage
  • DNA, Fungal (metabolism)
  • G2 Phase (drug effects)
  • Interphase (drug effects)
  • Mad2 Proteins
  • Microtubules (metabolism)
  • Mitosis (drug effects)
  • Molecular Sequence Data
  • Nuclear Export Signals
  • Nuclear Proteins (metabolism)
  • Recombinant Fusion Proteins (metabolism)
  • Schizosaccharomyces (cytology, drug effects, metabolism)
  • Schizosaccharomyces pombe Proteins (chemistry, metabolism)
  • Signal Transduction (drug effects)
  • Spindle Apparatus (drug effects, metabolism)

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