Magnesium and posthypoxic changes of nitrergic population in rat hippocampus.

Abstract

We used NADPH-diaphorase staining to study effects of magnesium pre-treatment during long-lasting hypoxia on the brain structure of rats. NADPH-diaphorase is an enzyme co-localized in neurons with NO-synthase that is responsible for NO synthesis. NO participates in hypoxic-ischaemic injury of the brain. Hypoxia was induced in consecutive days from the 2nd till the 11th day of postnatal life in a hypobaric chamber (for 8 hours per day). Magnesium was administered before each hypoxia exposition. At the age of 12 days, the animals were transcardially perfused with 4% buffered neutral paraformaldehyde under the deep thiopental anaesthesia. Cryostat sections were stained to identify NADPH-diaphorase positive neurons that were then quantified in five hippocampal regions. In comparison to the control animals, intermittent hypoxia brought about higher density of NADPH-diaphorase positive neurons in all studied areas of the hippocampal structure: in CA1 and CA3 areas of the hippocampus and in hilus, in the dorsal and ventral blades of the dentate gyrus. Magnesium pre-treatment during hypoxia reduced number of NADPH-diaphorase positive neurons in all studied areas.

• Authors: K Jandová, V Riljak, D Maresová, M Langmeier, J Pokorný
• Journal: Prague medical report (Prague Med Rep) Vol. 111 Issue 1 Pg. 55-64 ( 2010) ISSN: 1214-6994 [Print] Czech Republic
• PMID: 20359438 (Publication Type: Journal Article)

Chemical References

• Nitric Oxide
• NADPH Dehydrogenase
• Magnesium

Topics

• Animals
• Hippocampus (drug effects, metabolism)
• Hypoxia (metabolism)
• Magnesium (pharmacology)
• Male
• NADPH Dehydrogenase (metabolism)
• Neurons (metabolism)
• Nitric Oxide (metabolism)
• Rats
• Rats, Wistar