Enteric reovirus infection stimulates peanut-specific IgG2a responses in a mouse food allergy model.

Abstract

IgE-mediated food allergies are an important cause of life-threatening hypersensitivity reactions. Orally administered peanut antigens mixed with the mucosal adjuvant cholera toxin (CT) induce a strong peanut extract (PE)-specific serum IgE response that is correlated with T-helper type 1 (Th1) and type 2 (Th2)-like T-cell responses. This study was conducted to determine if respiratory enteric orphan virus (reovirus), a non-pathogenic virus that induces robust Th1-mediated mucosal and systemic responses could modulate induction of PE-specific allergic responses when co-administered with PE. Young mice were orally exposed to PE mixed with CT, reovirus, or both CT and reovirus. As expected, CT promoted PE-specific serum IgE, IgG1, and IgG2a and intestinal IgA production as well as splenic Th1- and Th2-associated cytokine recall responses. Reovirus did not alter PE-specific serum IgE and IgG1 levels, but substantially increased the PE-specific IgG2a response when co-administered with PE with or without CT. Additionally, reovirus significantly decreased the percentage of the Peyer's patch CD8+ T-cells and Foxp3+CD4+ T-regulatory cells when co-administered with PE. These results demonstrate that an acute mucosal reovirus infection and subsequent Th1 immune response is capable of modulating the Th1/Th2 controlled humoral response to PE. The reovirus-mediated increase in the PE-specific IgG2a antibody response may have therapeutic implications as increased levels of non-allergenic PE-specific IgG2a could block PE antigens from binding to IgE-sensitized mast cells.

• Authors: Ronald J Fecek, Marisa Marcondes Rezende, Ryan Busch, Ine Hassing, Raymond Pieters, Christopher F Cuff
• Journal: Immunobiology (Immunobiology) Vol. 215 Issue 12 Pg. 941-8 (Dec 2010) ISSN: 1878-3279 [Electronic] Netherlands
• PMID: 20356650 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010 Elsevier GmbH. All rights reserved.

Chemical References

• Cytokines
• Immunoglobulin A
• Immunoglobulin G
• Plant Extracts
• Cholera Toxin

Topics

• Animals
• Arachis (chemistry, immunology)
• CD8-Positive T-Lymphocytes (drug effects, metabolism, microbiology)
• Cell Line
• Cells, Cultured
• Cholera Toxin (immunology, pharmacology)
• Cytokines (immunology, metabolism)
• Disease Models, Animal
• Female
• Flow Cytometry
• Humans
• Immunoglobulin A (immunology)
• Immunoglobulin G (immunology)
• Intestinal Mucosa (drug effects, immunology, virology)
• Intestine, Small (drug effects, immunology, virology)
• Mice
• Mice, Inbred C3H
• Peanut Hypersensitivity (immunology)
• Peyer's Patches (immunology, virology)
• Plant Extracts (immunology, pharmacology)
• Reoviridae Infections (immunology)
• Spleen (cytology, drug effects, metabolism)
• Th1 Cells (drug effects, immunology)
• Tissue Culture Techniques