Cytochrome P450 family 4 in a cockroach: molecular cloning and regulation by regulation by hypertrehalosemic hormone.

Abstract

Hypertrehalosemic hormone (a carbohydrate-mobilizing neuroendocrine decapeptide) and starvation markedly increased levels of a cockroach (Blaberus discoidalis) fat body cytochrome P450 message. The gene represented by the cloned P450 cDNA has been named CYP4C1 (cytochrome P450 family 4, subfamily C, gene 1), a newly identified member of the ubiquitous cytochrome P450 monooxygenase gene superfamily. Blaberus CYP4C1 (511 amino acids, Mr = 58,485) has a hydrophobic NH2 terminus and a sequence near the COOH terminus that is homologous to the cysteine-containing heme-binding region definitive of cytochromes P450. The cockroach sequence is 32-36% identical to mammalian family 4A and 4B enzymes. It contains a 13-residue sequence characteristic of family 4 but not other P450s. This study suggests that CYP4C1 is hormonally regulated in association with energy substrate mobilization and supports the idea that family 4 is an old and widespread gene family.

• Authors: J Y Bradfield, Y H Lee, L L Keeley
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 88 Issue 10 Pg. 4558-62 (May 15 1991) ISSN: 0027-8424 [Print] United States
• PMID: 2034694 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Insect Hormones
• Neuropeptides
• hypertrehalosemic neuropeptide
• DNA
• Cytochrome P-450 Enzyme System

Topics

• Amino Acid Sequence
• Animals
• Base Sequence
• Cloning, Molecular
• Cockroaches (genetics)
• Cytochrome P-450 Enzyme System (genetics)
• DNA (genetics, isolation & purification)
• Gene Expression Regulation (drug effects)
• Humans
• Insect Hormones (pharmacology)
• Male
• Molecular Sequence Data
• Neuropeptides (pharmacology)
• Nucleic Acid Hybridization
• Rats
• Sequence Homology, Nucleic Acid