Truncated hantavirus nucleocapsid proteins for serotyping Sin Nombre, Andes, and Laguna Negra hantavirus infections in humans and rodents.

Abstract

Sin Nombre virus (SNV), Andes virus (ANDV), and Laguna Negra virus (LANV) have been known as the dominant causative agents of hantavirus pulmonary syndrome (HPS). ANDV and LANV, with different patterns of pathogenicity, exist in a sympatric relationship. Moreover, there is documented evidence of person-to-person transmission of ANDV. Therefore, it is important in clinical medicine and epidemiology to know the serotype of a hantavirus causing infection. Truncated SNV, ANDV, and LANV recombinant nucleocapsid proteins (trNs) missing 99 N-terminal amino acids (trN100) were expressed using a baculovirus system, and their applicability for serotyping SNV, ANDV, and LANV infection by the use of enzyme-linked immunosorbent assays (ELISA) was examined. HPS patient sera and natural-reservoir rodent sera infected with SNV, ANDV, and LANV showed the highest optical density (OD) values for homologous trN100 antigens. Since even patient sera with lower IgM and IgG antibody titers were serotyped, the trN100s are therefore considered useful for serotyping with early-acute-phase sera. In contrast, assays testing whole recombinant nucleocapsid protein antigens of SNV, ANDV, and LANV expressed in Escherichia coli detected homologous and heterologous antibodies equally. These results indicated that a screening ELISA using an E. coli-expressed antigen followed by a serotyping ELISA using trN100s is useful for epidemiological surveillance in regions where two or more hantavirus species cocirculate.

• Authors: Takaaki Koma, Kumiko Yoshimatsu, Noemi Pini, David Safronetz, Midori Taruishi, Silvana Levis, Rika Endo, Kenta Shimizu, Shumpei P Yasuda, Hideki Ebihara, Heinz Feldmann, Delia Enria, Jiro Arikawa
• Journal: Journal of clinical microbiology (J Clin Microbiol) Vol. 48 Issue 5 Pg. 1635-42 (May 2010) ISSN: 1098-660X [Electronic] United States
• PMID: 20335425 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• Antigens, Viral
• Immunoglobulin G
• Immunoglobulin M
• Nucleocapsid Proteins
• Recombinant Proteins

Topics

• Animals
• Antibodies, Viral (blood)
• Antigens, Viral (genetics)
• Baculoviridae (genetics)
• Enzyme-Linked Immunosorbent Assay (methods)
• Escherichia coli (genetics)
• Gene Expression
• Genetic Vectors
• Orthohantavirus (classification, isolation & purification)
• Hantavirus Infections (diagnosis, veterinary, virology)
• Humans
• Immunoglobulin G (blood)
• Immunoglobulin M (blood)
• Mass Screening (methods)
• Nucleocapsid Proteins (genetics)
• Recombinant Proteins (genetics)
• Rodent Diseases (diagnosis, virology)
• Rodentia
• Serotyping (methods)