Glucosamine regulation of LPS-mediated inflammation in human bronchial epithelial cells.

Abstract

Inflammation is a complex process involving cytokine production to regulate host defense cascades in order to clear pathogenic agents. Upregulation of inflammatory cytokines, such as IL-6 and IL-8 by bacteria infection, occurs in pulmonary tissues and has been demonstrated to be critical to the lung inflammatory response. Glucosamine, primarily identified as an anti-arthritis supplement, has been also regarded as a potential anti-inflammatory agent. Thus we hypothesized that lipopolysaccharide (LPS) would activate IL-6 and IL-8 expressions in human primary bronchial epithelial cells and glucosamine could attenuate such an effect. The RT-PCR, real-time PCR, and ELISA analyses demonstrated that LPS-induced mRNAs encoding IL-6 and IL-8 and the subsequent secretion of IL-6 and IL-8 were inhibited by glucosamine treatment. MTT, alamarBlue, and annexin V apoptosis assays all suggested that this inhibition effect was not due to a cytotoxic effect mediated by glucosamine. Using the inhibitors of the MAP kinases and NFkappaB, it was revealed that p38, JNK and ERK, as well as NFkappaB, are all involved in LPS-induced IL-8 secretion; however only p38 is involved in LPS-induced IL-6 secretion. Immunoblot analysis further demonstrated that LPS-mediated phosphorylation of JNK and ERK, but not the LPS-induced NFkappaB translocation, was inhibited by glucosamine. Altogether, our results indicate that glucosamine can potently suppress LPS-induced inflammatory cytokine expression, at least in part via attenuation of MAPK activation.

• Authors: Yuh-Lin Wu, Yu Ru Kou, Hui-Ling Ou, Han-Yun Chien, Kun-Han Chuang, Han-Hsun Liu, Tzong-Shyuan Lee, Cheng-Yen Tsai, Meng-Lun Lu
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 635 Issue 1-3 Pg. 219-26 (Jun 10 2010) ISSN: 1879-0712 [Electronic] Netherlands
• PMID: 20307528 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-6
• Interleukin-8
• Lipopolysaccharides
• RNA, Messenger
• Glucosamine

Topics

• Bronchi (pathology)
• Cell Survival (drug effects)
• Epithelial Cells (drug effects, metabolism, pathology)
• Gene Expression Regulation (drug effects)
• Glucosamine (pharmacology, therapeutic use)
• Humans
• Inflammation (drug therapy)
• Interleukin-6 (genetics, metabolism)
• Interleukin-8 (genetics, metabolism)
• Intracellular Space (drug effects, metabolism)
• Lipopolysaccharides (pharmacology)
• Promoter Regions, Genetic (genetics)
• RNA, Messenger (genetics, metabolism)
• Signal Transduction (drug effects)