Abstract | BACKGROUND:
Parathyroid hormone (PTH) is an active stimulator of bone marrow osteoblasts; it is involved in the niche organization, ie the bone marrow microenvironment which controls the turnover and the fate of endothelial progenitor cells (EPCs). PTH stimulates EPC production; this action can be measured by counting the number of circulating CD34+ cells. METHODS: This observational cross-sectional study aimed to verify this effect in 3 groups of hemodialysis patients with different serum PTH levels. The first group consisted of 11 patients affected by secondary hyperparathyroidism (SHPTH group, serum PTH levels >500 pg/ml); the second group consisted of 10 patients with serum PTH levels between 150 and 500 pg/ml (TargetPTH group); the third group consisted of 10 patients with serum PTH levels below the treatment target after parathyroidectomy (PTx group, serum PTH levels <150 pg/ml). Serum PTH, calcium (Ca), phosphorus (P), alkaline phosphatases (ALP), urea nitrogen, albumin and hemoglobin were measured. Flow cytofluorimetry with CD45+ sequential gating was effected; therefore, CD34+ cells could be analyzed. RESULTS: The SHPTH group showed significantly higher values of serum PTH, P and ALP (respectively, p<0.0001, p<0.033 and p<0.0001), and significantly lower values of circulating CD34+ cells (both in absolute and percent terms) in the SHPTH and in the TargetPTH groups (for both, p<0.0001). Two models of multiple regression analysis built with circulating CD34+ cells (expressed as percentage in the first one and as absolute values in the second one) as dependent variables showed that only serum PTH and P values were inversely associated with both. CONCLUSIONS: Our data suggest that an inverse relationship exists in uremic patients among circulating CD34+ cells and serum P and PTH levels. The count of circulating CD34+ cells might represent a novel biomarker for the assessment of the cardiovascular risk for dialysis patients.
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Authors | Carlo Lomonte, Clorinda Derosa, Luigi Vernaglione, Francesco Casucci, Nicola Losurdo, Pasquale Libutti, Annalisa Teutonico, Carlo Basile |
Journal | Journal of nephrology
(J Nephrol)
2010 Nov-Dec
Vol. 23
Issue 6
Pg. 693-8
ISSN: 1121-8428 [Print] Italy |
PMID | 20301083
(Publication Type: Journal Article)
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Chemical References |
- Antigens, CD34
- Parathyroid Hormone
- Phosphates
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Topics |
- Adult
- Aged
- Antigens, CD34
(analysis)
- Cell Count
- Cross-Sectional Studies
- Endothelial Cells
(cytology)
- Female
- Humans
- Male
- Middle Aged
- Parathyroid Hormone
(blood)
- Phosphates
(blood)
- Stem Cells
(cytology)
- Uremia
(blood)
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