Abstract |
We evaluated the feasibility and usefulness of reverse transcriptase-polymerase chain reaction (RT-PCR) on fine-needle aspirates for categorization of small blue round cell tumors (SBRCTs). A total of 51 cases, including 25 Ewing sarcoma/ peripheral primitive neuroectodermal tumors ( PNETs), 11 rhabdomyosarcomas, 13 neuroblastomas, and 2 desmoplastic small round cell tumors (DSRCTs) were analyzed. The detection of the EWS-FLI1 (20/25) and EWS-ERG (4/25) fusion transcripts resolved 24 of 25 cases of Ewing sarcoma/ PNET. The PAX3/7-FKHR fusion transcript was detected in 2 of 4 cases of alveolar rhabdomyosarcoma and the EWS-WT1 transcript in both cases of DSRCT. Tyrosine hydroxylase and 3,4-dihydroxyphenylalanine ( dopa) decarboxylase transcripts were demonstrated in 10 of 13 cases of neuroblastoma. In comparison, immunocytochemical analysis resolved 19 (76%) of 25 Ewing sarcomas, 9 (82%) of 11 rhabdomyosarcomas, 6 (46%) of 13 neuroblastomas, and 1 (50%) of 2 DSRCTs. Overall, RT-PCR resolved 38 (86%) of 44 vs 35 (69%) of 51 cases by immunocytochemical analysis. RT-PCR is easily applied to fine-needle aspirates of SBRCT and greatly facilitates accurate tumor typing.
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Authors | Upasana Gautam, Radhika Srinivasan, Arvind Rajwanshi, Deepak Bansal, Ram Kumar Marwaha, Rakesh Kumar Vasishtha |
Journal | American journal of clinical pathology
(Am J Clin Pathol)
Vol. 133
Issue 4
Pg. 633-45
(Apr 2010)
ISSN: 1943-7722 [Electronic] England |
PMID | 20231617
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Antineoplastic Combined Chemotherapy Protocols
(therapeutic use)
- Biopsy, Fine-Needle
(methods)
- Humans
- Neuroblastoma
(diagnosis, drug therapy, genetics)
- Neuroectodermal Tumors, Primitive, Peripheral
(diagnosis, drug therapy, genetics)
- Reverse Transcriptase Polymerase Chain Reaction
(methods)
- Rhabdomyosarcoma
(diagnosis, drug therapy, genetics)
- Sarcoma, Ewing
(diagnosis, drug therapy, genetics)
- Sarcoma, Small Cell
(diagnosis, drug therapy, genetics)
- Soft Tissue Neoplasms
(diagnosis, drug therapy, genetics)
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