Abstract |
A single chain glycoprotein with an estimated molecular mass of 160 kD (gp160) was previously identified as a human lung tumor-associated antigen. This tumor marker is shown here to be associated noncovalently with a second 130-kD protein. Sequential immunoprecipitation studies of surface iodinated lung tumor cell lysates reveal that this heterodimeric complex is indistinguishable serologically and structurally from the integrin VLA-2, found originally on activated T lymphocytes and platelets. The VLA-2-like complex expressed on the lung tumors possesses similar characteristic Mg2+ dependent binding of collagen and laminin as observed with VLA-2 on normal cells. RNA analysis indicates that human lung tumors express at least 20 times more VLA-2 alpha chain message than normal adult human lung tissue. The results presented here raise the possibility that the overproduction of VLA-2 may be involved in the pathogenesis of human lung tumors by modulating the invasive and metastatic potential of the tumor.
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Authors | F A Chen, E A Repasky, R B Bankert |
Journal | The Journal of experimental medicine
(J Exp Med)
Vol. 173
Issue 5
Pg. 1111-9
(May 01 1991)
ISSN: 0022-1007 [Print] United States |
PMID | 2022922
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antigens, Tumor-Associated, Carbohydrate
- Biomarkers, Tumor
- Cell Adhesion Molecules
- Extracellular Matrix Proteins
- Laminin
- Receptors, Very Late Antigen
- Collagen
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Topics |
- Antigens, Tumor-Associated, Carbohydrate
(analysis, genetics, metabolism)
- Biomarkers, Tumor
(analysis)
- Blotting, Northern
- Cell Adhesion Molecules
(analysis, genetics, metabolism)
- Collagen
(metabolism)
- Electrophoresis, Polyacrylamide Gel
- Extracellular Matrix Proteins
(chemistry, metabolism)
- Gene Expression Regulation, Neoplastic
(physiology)
- Humans
- Laminin
(metabolism)
- Lung Neoplasms
(etiology, metabolism, pathology)
- Neoplasm Metastasis
(physiopathology)
- Precipitin Tests
- Receptors, Very Late Antigen
(analysis, genetics, metabolism)
- Tumor Cells, Cultured
(metabolism, pathology, ultrastructure)
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