Abstract | BACKGROUND: OBJECTIVES: METHODS: Using offspring from mice with experimentally induced EBA, we examined retention times of diaplacentally transmitted autoantibodies to type VII collagen in serum of neonatal mice by enzyme-linked immunosorbent assay and of immunoreactant deposits in skin by direct immunofluorescence microscopy. Additionally, the pathogenic potential of transmitted autoantibodies was evaluated in descendant mice. RESULTS: Immediately after birth, comparable levels of pathogenic antibody concentrations were observed in maternal and neonatal mice. The clearance time of skin-bound autoantibodies was twice as long as that of circulating autoantibodies (8 and 4 weeks, respectively). Maternofetal transfer of pathogenic autoantibodies produced specific immunopathological ( IgG, IgG1, IgG2a/b and complement C3 deposits) but not histological or clinical alterations in skin of offspring mice. CONCLUSIONS: Although still to be confirmed in humans, our findings add to the knowledge on turnover rates of circulating and skin-bound autoantibodies in autoimmune bullous dermatoses, which in turn may facilitate a more specific monitoring of these antibodies during the disease course, reduce the need for repeated skin biopsies, and may also be helpful in guiding treatment decisions.
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Authors | M Kasperkiewicz, M Hirose, A Recke, E Schmidt, D Zillikens, R J Ludwig |
Journal | The British journal of dermatology
(Br J Dermatol)
Vol. 162
Issue 5
Pg. 1064-70
(May 2010)
ISSN: 1365-2133 [Electronic] England |
PMID | 20222918
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Autoantibodies
- Collagen Type VII
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Topics |
- Animals
- Autoantibodies
(blood, metabolism)
- Collagen Type VII
(immunology)
- Complement Activation
(immunology)
- Epidermolysis Bullosa Acquisita
(immunology)
- Female
- Male
- Maternal-Fetal Exchange
(immunology)
- Mice
- Microscopy, Fluorescence
- Phenotype
- Pregnancy
- Pregnancy Complications
(immunology)
- Skin
(immunology)
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