Our aim was to improve a method for detecting
respiratory hypersensitivity by testing three confirmed respiratory
allergens (
trimellitic anhydride [TMA],
phthalic anhydride [PA] and
toluene diisocyanate [TDI]), an environmental chemical of uncertain allergenicity (2,4-d-butyl [DB]), a confirmed contact
allergen (
2,4-dinitrochlorobenzene [
DNCB]) and a standard
irritant (
sodium dodecyl sulfate [SDS]). BALB/c mice were topically sensitized (nine times in 3 weeks) with these chemicals, then challenged via the trachea. One day post-challenge, samples were taken from the mice to assay for total
immunoglobulin (
IgE and
IgG(1)) levels in serum and bronchoalveolar lavage fluid (BALF); differential cell counts and
cytokine/
chemokine levels in BALF; lymphocyte counts and
surface antigen expression on B-cells within lung-associated lymph nodes (LNs); ex situ
cytokine production by cells from these LNs; and gene expression in BALF (CCR3) and LNs (CCR4, STAT6 and GATA-3). The three confirmed respiratory
allergens and DB induced immune response characteristic of immediate-type respiratory reactions, as evidenced by increased total
IgE and
IgG(1) levels; influx of eosinophils, neutrophils,
chemokines and
cytokines in BALF; increased
surface antigen expression on B-cells in LNs; increased Th2
cytokine production in LNs; and increased respiratory
allergy-related gene expression in both BALF and LNs. In contrast,
DNCB and SDS treatments yielded, at most, insignificant increases in all respiratory allergic parameters. Thus, the protocol was equally suitable for use with an environmental chemical of unknown allergenicity and for typical respiratory
allergens. Since the protocol differentiated respiratory
allergens from contact
allergens and irritants, it may be useful for detecting respiratory
allergy related to environmental chemicals.