Abstract |
RNA interference by short interfering RNAs (siRNAs) holds promise as a therapeutic strategy, but use of siRNAs in vivo remains limited. Here, we developed a system to target delivery of siRNAs to glomeruli via poly( ethylene glycol)-poly( l-lysine)-based vehicles. The siRNA/nanocarrier complex was approximately 10 to 20 nm in diameter, a size that would allow it to move across the fenestrated endothelium to access to the mesangium. After intraperitoneal injection of fluorescence-labeled siRNA/nanocarrier complexes, we detected siRNAs in the blood circulation for a prolonged time. Repeated intraperitoneal administration of a mitogen-activated protein kinase 1 (MAPK1) siRNA/nanocarrier complex suppressed glomerular MAPK1 mRNA and protein expression in a mouse model of glomerulonephritis; this improved kidney function, reduced proteinuria, and ameliorated glomerular sclerosis. Furthermore, this therapy reduced the expression of the profibrotic markers TGF-beta1, plasminogen activator inhibitor-1, and fibronectin. In conclusion, we successfully silenced intraglomerular genes with siRNA using nanocarriers. This technique could aid the investigation of molecular mechanisms of renal disease and has potential as a molecular therapy of glomerular diseases.
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Authors | Hideki Shimizu, Yuichi Hori, Shinya Kaname, Koei Yamada, Nobuhiro Nishiyama, Satoru Matsumoto, Kanjiro Miyata, Makoto Oba, Akira Yamada, Kazunori Kataoka, Toshiro Fujita |
Journal | Journal of the American Society of Nephrology : JASN
(J Am Soc Nephrol)
Vol. 21
Issue 4
Pg. 622-33
(Apr 2010)
ISSN: 1533-3450 [Electronic] United States |
PMID | 20203158
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- RNA, Small Interfering
- Mapk1 protein, mouse
- Mitogen-Activated Protein Kinase 1
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Topics |
- Animals
- Genetic Therapy
(methods)
- Glomerulonephritis
(genetics, therapy)
- Kidney Glomerulus
- Mice
- Mitogen-Activated Protein Kinase 1
(genetics)
- RNA, Small Interfering
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