Bactericidal and virucidal ultrathin films assembled layer by layer from polycationic N-alkylated polyethylenimines and polyanions.

Abstract

In this work, we designed contact-killing ionically cross-linked polymeric thin films using Layer-by-Layer (LbL) technology. A polycation, N,N-dodecyl,methyl-polyethylenimine, with microbicidal activity was layered with a polyanion, such as poly(acrylic acid), to create LbL films highly effective against both airborne and waterborne Escherichia coli and Staphylococcus aureus (Gram negative and positive bacteria, respectively), as well as influenza A/WSN (H1N1) virus. The dependence of the microbicidal activity on the pH during and post-assembly of LbL film formation, the nature of the polycation and polyanion, the number of layers in the LbL film, and other experimental variables was investigated quantitatively.

• Authors: Sze Yinn Wong, Qing Li, Jovana Veselinovic, Byeong-Su Kim, Alexander M Klibanov, Paula T Hammond
• Journal: Biomaterials (Biomaterials) Vol. 31 Issue 14 Pg. 4079-87 (May 2010) ISSN: 1878-5905 [Electronic] Netherlands
• PMID: 20163855 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
• Copyright: Copyright 2010 Elsevier Ltd. All rights reserved.

Chemical References

• Anti-Bacterial Agents
• Antiviral Agents
• N,N-dodecyl,methyl-polyethylenimine
• Polyamines
• Polyelectrolytes
• Polymers
• polyanions
• polycations
• Polyethyleneimine

Topics

• Air Microbiology
• Animals
• Anti-Bacterial Agents (pharmacology)
• Antiviral Agents (pharmacology)
• Cell Adhesion (drug effects)
• Cell Death (drug effects)
• Cell Line
• Escherichia coli (drug effects)
• Mice
• Microbial Sensitivity Tests
• Microscopy, Electron, Scanning
• Molecular Conformation
• Orthomyxoviridae (drug effects)
• Polyamines (chemistry, pharmacology)
• Polyelectrolytes
• Polyethyleneimine (analogs & derivatives, chemistry, pharmacology)
• Polymers (chemistry, pharmacology)
• Staphylococcus aureus (drug effects)
• Water Microbiology