Detection of human T-cell leukemia virus type I proviral sequences from fixed tissues of seropositive patients.

Abstract

Fixed lymphoma tissues from 11 patients seropositive for human T-cell leukemia virus type I (HTLV-I) antibodies were analyzed for proviral sequences by the polymerase chain reaction. Typical adult T-cell leukemia/lymphomas (ATLLs) from nine patients were positive for HTLV-I sequences. In contrast, one of two lymphomas with immunophenotypes atypical for ATLL was negative for HTLV-I. This HTLV-I DNA-negative lymphoma, although present in an HTLV-I-seropositive patient, was therefore reclassified. The HTLV-I tax gene was always detected in the ATLL tissues, whereas segments of the pol gene were not detected in half the cases. These studies demonstrate that fixed non-ATLL specimens can be distinguished from ATLL specimens.

• Authors: D Shibata, M Tokunaga, N Sasaki, K Nanba
• Journal: American journal of clinical pathology (Am J Clin Pathol) Vol. 95 Issue 4 Pg. 536-9 (Apr 1991) ISSN: 0002-9173 [Print] England
• PMID: 2014780 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• DNA, Viral

Topics

• Adult
• Aged
• DNA, Viral (genetics)
• Diagnosis, Differential
• Female
• Genes, pX (genetics)
• Genes, pol (genetics)
• HIV Seropositivity (genetics, microbiology)
• Human T-lymphotropic virus 1 (genetics, isolation & purification)
• Humans
• Immunophenotyping
• Leukemia-Lymphoma, Adult T-Cell (diagnosis, genetics, microbiology)
• Male
• Middle Aged
• Polymerase Chain Reaction
• Proviruses (genetics, isolation & purification)
• Serologic Tests