Eotaxin-2/CCL24 and eotaxin-3/CCL26 exert differential profibrogenic effects on human lung fibroblasts.

Abstract

BACKGROUND:

Eotaxin-2/CCL24 and eotaxin-3/CCL26 play an important role in eosinophil chemotaxis and activation in asthma. We previously demonstrated that eotaxin/CCL11 is profibrogenic for human lung fibroblasts. The effect of eotaxin-2/ CCL24 and eotaxin-3/CCL26 on lung fibroblasts has not yet been investigated.

OBJECTIVE:

To evaluate whether eotaxin-2/CCL24 and eotaxin-3/CCL26 modulate fibrotic properties of lung fibroblasts.

METHODS:

Fibroblast proliferation was evaluated by means of 3-hydroxythymidine incorporation. Collagen production was assessed by means of 3-hydroxyproline incorporation and biochemical staining. Chemotaxis was determined using Boyden chambers. Expression of alpha-smooth muscle actin was evaluated by means of immunostaining. Transforming growth factor beta1 release was assessed using enzyme-linked immunosorbent assay. Parametric analysis of variance, followed by the Tukey-Kramer multiple comparisons test, was used to calculate statistical significance.

RESULTS:

Eotaxin-2/CCL24 but not eotaxin-3/CCL26 stimulated human lung fibroblast proliferation and collagen synthesis. In contrast, eotaxin-3/CCL26 but not eotaxin-2/CCL24 promoted fibroblast migration. Neither eotaxin-2/CCL24 nor eotaxin-3/ CCL26 induced the expression of alpha-smooth muscle actin or transforming growth factor beta1 from lung fibroblasts.

CONCLUSIONS:

Eotaxin-2/CCL24 and eotaxin-3/CCL26 have differential profibrogenic effects on human lung fibroblasts. These CC chemokines may, therefore, contribute to airway remodeling in asthma.

• Authors: Martin Kohan, Ilaria Puxeddu, Reuven Reich, Francesca Levi-Schaffer, Neville Berkman
• Journal: Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology (Ann Allergy Asthma Immunol) Vol. 104 Issue 1 Pg. 66-72 (Jan 2010) ISSN: 1081-1206 [Print] United States
• PMID: 20143648 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Actins
• CCL24 protein, human
• CCL26 protein, human
• Chemokine CCL24
• Chemokine CCL26
• Chemokines, CC
• Recombinant Proteins
• Transforming Growth Factor beta1
• Collagen

Topics

• Actins (biosynthesis)
• Airway Remodeling
• Cell Differentiation (drug effects)
• Cell Line
• Cell Proliferation (drug effects)
• Chemokine CCL24 (pharmacology)
• Chemokine CCL26
• Chemokines, CC (pharmacology)
• Chemotaxis (drug effects)
• Collagen (biosynthesis)
• Fibroblasts (drug effects, metabolism, pathology)
• Humans
• Lung (pathology)
• Pulmonary Fibrosis (etiology, pathology)
• Recombinant Proteins (pharmacology)
• Transforming Growth Factor beta1 (metabolism)