Abstract | BACKGROUND: METHODS: RESULTS: At 12 weeks, CLA-TG and CLA-FFA attenuated the increase in ACFs induced by 10% beef tallow and AOM pretreatment. At 44 weeks, both forms of CLA attenuated multiple colon cancers, and CLA-FFA reduced the incidence of colon cancer to 50% of that seen with CLA-TG. CLA-TG and CLA-FFA decreased the number of 5-bromo-2'-deoxyuridine-positive cells in AOM-pretreated rats fed with 10% beef tallow. CLA-FFA increased the number of apoptotic cells and the activity of caspase-3 in the colon mucosa, and CLA-TG enhanced the activity of caspase-3. Both forms of CLA suppressed Wnt signaling and the arachidonic acid cascade in rats treated with beef tallow and AOM. CONCLUSION: These results suggested that CLA-TG and CLA-FFA suppressed colon carcinogenesis in rats with long-term feeding of a 10% beef tallow diet, through several mechanisms. The results of the present study with rats might be applicable to humans.
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Authors | Ryosuke Shiraishi, Ryuichi Iwakiri, Takehiro Fujise, Tsukasa Kuroki, Takashi Kakimoto, Tooru Takashima, Yasuhisa Sakata, Seiji Tsunada, Yutaka Nakashima, Teruyoshi Yanagita, Kazuma Fujimoto |
Journal | Journal of gastroenterology
(J Gastroenterol)
Vol. 45
Issue 6
Pg. 625-35
(Jun 2010)
ISSN: 1435-5922 [Electronic] Japan |
PMID | 20143104
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Dietary Fats
- Fats
- Linoleic Acids, Conjugated
- Wnt Proteins
- Arachidonic Acid
- tallow
- Azoxymethane
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Topics |
- Animals
- Apoptosis
(drug effects)
- Arachidonic Acid
(metabolism)
- Azoxymethane
(toxicity)
- Cattle
- Cell Proliferation
(drug effects)
- Colonic Neoplasms
(etiology, prevention & control)
- Dietary Fats
(toxicity)
- Fats
(toxicity)
- Linoleic Acids, Conjugated
(pharmacology)
- Male
- Precancerous Conditions
(etiology)
- Rats
- Rats, Sprague-Dawley
- Signal Transduction
(drug effects)
- Time Factors
- Wnt Proteins
(drug effects, metabolism)
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