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[Effects of nuclear factor-kappaB siRNA upon dextran sulphate sodium-induced colitis murine model].

AbstractOBJECTIVE:
To investigate the effects of nuclear factor-kappaB (NF-kappaB) siRNA upon dextran sulphate sodium (DSS)-induced colitis.
METHODS:
Thirty-six BALB/C mice were randomly divided into 4 groups: normal control group, NF-kappaB siRNA, scrambled siRNA and DSS group. Colitis was induced by treatment with 5% DSS in drinking water and evaluated by disease activity index (DAI) and histological score. The tumor necrosis factor (TNF-alpha) level of colonic mucosa was measured by enzyme linked immunosorbent assay (ELISA). NF-kappaB p65 expression was determined by immunohistochemical staining.
RESULTS:
Compared with the scrambled siRNA (DAI 3.42 +/- 0.67, histological score 4.65 +/- 1.53) and DSS group (3.73 +/- 0.55, 4.47 +/- 1.52), a significant decrease was observed in DAI (2.31 +/- 0.26) and histological score (2.19 +/- 0.77) in mice with NF-kappaB p65 siRNA (both P < 0.05). The expression of NF-kappaB p65 and TNF-alpha [(266 +/- 35) pg/ml] in mice with DSS-induced colitis was significantly reduced after treatment with NF-kappaB p65 siRNA (P < 0.05) in comparisons with the scrambled siRNA [(412 +/- 51) pg/ml] and DSS group pg/ml [(449 +/- 44) pg/ml].
CONCLUSION:
NF-kappaB p65siRNA shows protective effects upon the mice with DSS-induced colitis. Blockade of NF-kappaB pathway by NF-kappaB p65 siRNA may serve as a novel gene therapy for ulcerative colitis.
AuthorsLi-na Cheng, Xiao-li Huang, Hua-tian Gan
JournalZhonghua yi xue za zhi (Zhonghua Yi Xue Za Zhi) Vol. 89 Issue 34 Pg. 2416-9 (Sep 15 2009) ISSN: 0376-2491 [Print] China
PMID20137697 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • NF-kappa B
  • RNA, Small Interfering
  • Tumor Necrosis Factor-alpha
  • Dextran Sulfate
Topics
  • Animals
  • Colitis, Ulcerative (metabolism)
  • Dextran Sulfate
  • Disease Models, Animal
  • Female
  • Intestinal Mucosa (metabolism)
  • Mice
  • Mice, Inbred BALB C
  • NF-kappa B (genetics, metabolism)
  • RNA, Small Interfering
  • Tumor Necrosis Factor-alpha (metabolism)

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