Radial shock waves effectively introduced NF-kappa B decoy into rat achilles tendon cells in vitro.

Abstract

The purpose of this study was to test if radial shock waves could enhance the introduction of nuclear factor-kappa B (NF-kappaB) decoy oligodeoxynucleotides, which is reported to markedly inhibit NF-kappaB activation and suppress pro-inflammatory cytokine gene expression, using rat Achilles tendon cells. In the presence of NF-kappaB decoy labeled with or without fluorescein isothiocyanate (FITC) in culture media, radial shock waves were applied to the tendon cells in variable conditions and cultivated for 24 h. The transfection rate was assessed by counting FITC-positive cells, and IL-1-induced NF-kappaB activation in the cells was assessed. Radial shock waves significantly enhanced introduction of NF-kappaB decoy-FITC into the tendon cells. IL-1-induced NF-kappaB activation was significantly inhibited by pretreatment of the cells with NF-kappaB decoy combined with radial shock wave exposure. The present study demonstrated the effectiveness of radial shock waves on introduction of NF-kappaB decoy into tendon cells. Radial shock wave treatment combined with local NF-kappaB decoy administration could be a novel therapeutic strategy for chronic tendinopathy.

• Authors: Kaori Sugioka, Koichi Nakagawa, Ryo Murata, Nobuyasu Ochiai, Takahisa Sasho, Momoko Arai, Hiroaki Tsuruoka, Seiji Ohtori, Takashi Saisu, Takefumi Gemba, Kazuhisa Takahashi
• Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society (J Orthop Res) Vol. 28 Issue 8 Pg. 1078-83 (Aug 2010) ISSN: 1554-527X [Electronic] United States
• PMID: 20135689 (Publication Type: Journal Article)
• Copyright: Copyright 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.

Chemical References

• NF-kappa B
• NF-kappaB decoy
• Oligodeoxyribonucleotides

Topics

• Achilles Tendon (metabolism)
• Animals
• Cells, Cultured
• Gene Transfer Techniques
• High-Energy Shock Waves
• NF-kappa B (metabolism)
• Oligodeoxyribonucleotides (metabolism)
• Rats
• Rats, Sprague-Dawley