Abstract |
The fungus Taiwanofungus camphoratus is commonly used for medicinal purposes in Taiwan. It is used as a detoxicant for food poisoning and considered to be a precious folk medicine for hepatoprotection and anti- inflammation. In this study, a lipopolysaccaride (LPS)-challenged ICR mouse acute inflammation model and a LPS-induced macrophage model were used to evaluate the anti-inflammatory activity of T. camphoratus. Ethanol extract of T. camphoratus significantly inhibited expression of iNOS and COX-2 in the liver of LPS-challenged acute inflammatory mice. The ethyl acetate fraction and its isolated compound, antrocamphin A, significantly suppressed nitrite/ nitrate concentration in LPS-challenged RAW 264.7 cells. Antrocamphin A showed potent anti-inflammatory activity by suppressing pro-inflammatory molecule release via the down-regulation of iNOS and COX-2 expression through the NF-kappaB pathway. This study, therefore, first demonstrates the bioactive compound of T. camphoratus and illustrates the mechanism by which it confers its anti-inflammatory activity.
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Authors | Yu-Hsin Hsieh, Fang-Hua Chu, Ya-Shin Wang, Shih-Chang Chien, Shang-Tzen Chang, Jei-Fu Shaw, Chieh-Yin Chen, Wen-Wei Hsiao, Yueh-Hsiung Kuo, Sheng-Yang Wang |
Journal | Journal of agricultural and food chemistry
(J Agric Food Chem)
Vol. 58
Issue 5
Pg. 3153-8
(Mar 10 2010)
ISSN: 1520-5118 [Electronic] United States |
PMID | 20128588
(Publication Type: Journal Article)
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Chemical References |
- Alkynes
- Anisoles
- Anti-Inflammatory Agents
- DNA Primers
- antrocamphin A
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Topics |
- Alkynes
(isolation & purification, pharmacology)
- Animals
- Anisoles
(isolation & purification, pharmacology)
- Anti-Inflammatory Agents
(isolation & purification, pharmacology)
- Base Sequence
- Basidiomycota
(chemistry)
- DNA Primers
- Fruiting Bodies, Fungal
(chemistry)
- Mice
- Mice, Inbred ICR
- Reverse Transcriptase Polymerase Chain Reaction
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