Abstract | OBJECTIVES: MATERIALS AND METHODS: Rats were fed semipurified diets with (n = 16) or without (n = 16) GGs (0.1% w/w of total lipid). After 2 weeks of feeding, animals were injected with saline (n = 8/diet group) or LPS (n = 8/diet group) (IP, 3 mg mL(-1) kg(-1)). Intestinal tissue, mucosa, and blood sample were collected 6 hours post-LPS exposure. The effect of dietary GGs on production/expression of IL-10, NO, inducible NO synthase, and occludin protein was determined. RESULTS: Dietary GGs increased IL-10 content in intestinal mucosa significantly by 32-fold (P < 0.0001) and in plasma by 2.4-fold (P < 0.001). Feeding animals a ganglioside-enriched diet decreased total NO content in intestinal mucosa and plasma by 44% and 30%, respectively, and inhibited inducible NO synthase expression following LPS exposure compared with control animals. Dietary GGs reduced the degradation of occludin tight junction protein in response to LPS. CONCLUSIONS:
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Authors | Eek J Park, Alan B R Thomson, Michael T Clandinin |
Journal | Journal of pediatric gastroenterology and nutrition
(J Pediatr Gastroenterol Nutr)
Vol. 50
Issue 3
Pg. 321-8
(Mar 2010)
ISSN: 1536-4801 [Electronic] United States |
PMID | 20118807
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Inflammatory Agents
- Dietary Fats
- Gangliosides
- Lipopolysaccharides
- Membrane Proteins
- OCLN protein, human
- Occludin
- Ocln protein, rat
- Interleukin-10
- Nitric Oxide
- Nitric Oxide Synthase Type II
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Topics |
- Acute Disease
- Animals
- Anti-Inflammatory Agents
(pharmacology)
- Cell Membrane Permeability
(drug effects)
- Dietary Fats
(administration & dosage)
- Gangliosides
(pharmacology)
- Inflammation
(chemically induced, drug therapy, metabolism)
- Interleukin-10
(blood, metabolism)
- Intestinal Absorption
- Intestinal Mucosa
(drug effects, metabolism)
- Lipopolysaccharides
- Male
- Membrane Proteins
(metabolism)
- Nitric Oxide
(blood, metabolism)
- Nitric Oxide Synthase Type II
(metabolism)
- Occludin
- Rats
- Rats, Sprague-Dawley
- Tight Junctions
(drug effects, metabolism)
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