Abstract |
HIV-specific CTL play an important role in the host control of HIV infection. HIV-nef may facilitate escape of HIV-infected cells from CTL recognition by selectively downregulating the expression of HLA-A and HLA-B molecules, while surface expression of HLA-C is unaffected. The HLA-C-restricted CTL responses have previously been largely ignored and poorly characterized. We examined the frequency, function, and phenotype of HLA-C-restricted CTL in ten antiretroviral therapy-naïve Caucasian and African individuals with chronic HIV-1 infection (for at least 8 years; CD4 cell counts in the range of 50-350) who carried the HLA-Cw04 allele. HLA-Cw04-restricted CTL that recognize a conserved epitope within HIV-1 envelope (aa 375-383 SF9) were analyzed using IFN-gamma ELISPOT assays and phenotypic analysis was carried out by flow cytometry. HLA-C-restricted CTL play an important role in the HIV-specific response, and can account for as much as 54% of the total response. HLA-C-restricted CTL are functionally and phenotypically identical to HLA-A- and HLA-B-restricted CTL. HLA-C-restricted CTL in chronic HIV infection are memory cells of an intermediate phenotype, characterized by high CD27 and low CD28 expression and lack of perforin production.
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Authors | Azure T Makadzange, Geraldine Gillespie, Tao Dong, Peter Kiama, Job Bwayo, Joshua Kimani, Frank Plummer, Philippa Easterbrook, Sarah L Rowland-Jones |
Journal | European journal of immunology
(Eur J Immunol)
Vol. 40
Issue 4
Pg. 1036-41
(Apr 2010)
ISSN: 1521-4141 [Electronic] Germany |
PMID | 20104487
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CD28 Antigens
- HIV Antigens
- HLA-C Antigens
- HLA-C*04 antigen
- Immunodominant Epitopes
- Tumor Necrosis Factor Receptor Superfamily, Member 7
- nef Gene Products, Human Immunodeficiency Virus
- nef protein, Human immunodeficiency virus 1
- Interferon-gamma
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Topics |
- Black People
(genetics)
- CD28 Antigens
(analysis)
- CD4 Lymphocyte Count
- Chronic Disease
- Cohort Studies
- Flow Cytometry
- HIV Antigens
(immunology)
- HIV Infections
(ethnology, immunology)
- HIV-1
- HLA-C Antigens
(genetics, immunology)
- Humans
- Immunodominant Epitopes
(immunology)
- Immunologic Memory
(immunology)
- Immunophenotyping
- Interferon-gamma
(metabolism)
- T-Lymphocyte Subsets
(immunology)
- T-Lymphocytes, Cytotoxic
(immunology)
- Tumor Necrosis Factor Receptor Superfamily, Member 7
(analysis)
- White People
(genetics)
- nef Gene Products, Human Immunodeficiency Virus
(physiology)
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