Loss of cannabinoid CB1 receptor expression in the 6-hydroxydopamine-induced nigrostriatal terminal lesion model of Parkinson's disease in the rat.

Abstract

The endocannabinoid system is emerging as a potential alternative to the dopaminergic system for the treatment of Parkinson's disease. Like all emerging targets, validation of this system's potential for treating human Parkinsonism necessitates testing in animal models of the condition. However, if components of the endocannabinoid system are altered by the induction of a Parkinsonian state in animal models, this could have an impact on the interpretation of such preclinical experiments. This study sought to determine if expression of the CB(1) subtype of cannabinoid receptor is altered in the two most commonly used rat models of Parkinson's disease. Parkinsonian lesions were induced by stereotaxic injection of 6-hydroxydopamine into the axons (medial forebrain bundle) or terminals (striatum) of the nigrostriatal pathway. On days 1, 3, 7, 14 and 28 post-lesion, rats were sacrificed and brains were processed for tyrosine hydroxylase and CB(1) receptor immunohistochemistry. The CB(1) receptor was expressed strongly in the substantia nigra pars reticulata, minimally overlapping with tyrosine hydroxylase immunoreactivity in the pars compacta. Interestingly, while there was little change in CB(1) receptor expression following axonal lesion, expression of the receptor was significantly reduced following terminal lesion. Loss of CB(1) receptor expression in the pars reticulata correlated significantly with the loss of striatal and nigral volume after terminal lesion indicating this may have been due to 6-hydroxydopamine-induced non-specific damage of striatonigral neurons which are known to express CB(1) receptors. Thus, this result has implications for the choice of model and interpretation of studies used to investigate potential cannabinoid-based therapies for Parkinson's disease as well as striatonigral diseases such as Huntington's disease and Multiple Systems Atrophy.

• Authors: Sinéad Walsh, Katarzyna Mnich, Ken Mackie, Adrienne M Gorman, David P Finn, Eilís Dowd
• Journal: Brain research bulletin (Brain Res Bull) Vol. 81 Issue 6 Pg. 543-8 (Apr 05 2010) ISSN: 1873-2747 [Electronic] United States
• PMID: 20097273 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2010 Elsevier Inc. All rights reserved.

Chemical References

• Adrenergic Agents
• Receptor, Cannabinoid, CB1
• Oxidopamine
• Tyrosine 3-Monooxygenase

Topics

• Adrenergic Agents (toxicity)
• Animals
• Axons (drug effects, metabolism, pathology)
• Corpus Striatum (drug effects, pathology)
• Disease Models, Animal
• Gene Expression
• Male
• Medial Forebrain Bundle (drug effects, pathology)
• Nerve Degeneration (chemically induced, metabolism, pathology)
• Neurons (drug effects, metabolism, pathology)
• Organ Size
• Oxidopamine (toxicity)
• Parkinson Disease
• Parkinsonian Disorders (chemically induced, metabolism, pathology)
• Random Allocation
• Rats
• Receptor, Cannabinoid, CB1 (genetics, metabolism)
• Substantia Nigra (metabolism, pathology)
• Time Factors
• Tyrosine 3-Monooxygenase (metabolism)