In vitro growth and single-leaf photosynthetic response of Cymbidium plantlets to super-elevated CO2 under cold cathode fluorescent lamps.
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| Abstract |
To examine the effectiveness of super-elevated (10,000 micromol mol(-1)) CO(2) enrichment under cold cathode fluorescent lamps (CCFL) for the clonal propagation of Cymbidium, plantlets were cultured on modified Vacin and Went (VW) medium under 0, 3,000 and 10,000 micromol mol(-1) CO(2) enrichment and two levels of photosynthetic photon flux density (PPFD, 45 and 75 micromol m(-2) s(-1)). Under high PPFD, 10,000 micromol mol(-1) CO(2) increased root dry weight and promoted shoot growth. In addition, a decrease in photosynthetic capacity and chlorosis at leaf tips were observed. Rubisco activity and stomatal conductance of these plantlets were lower than those of plantlets at 3,000 micromol mol(-1) CO(2) under high PPFD, which had a higher photosynthetic capacity. On the other hand, plantlets on Kyoto medium grown in 10,000 micromol mol(-1) CO(2) under high PPFD had a higher photosynthetic rate than those on modified VW medium; no chlorosis was observed. Furthermore, growth of plantlets, in particular the roots, was remarkably enhanced. This result indicates that a negative response to super-elevated CO(2) under high PPFD could be improved by altering medium components. Super-elevated CO(2) enrichment of in vitro-cultured Cymbidium could positively affect the efficiency and quality of commercial production of clonal orchid plantlets.
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| Authors | Atsushi Norikane, Takejiro Takamura, Masahiro Morokuma, Michio Tanaka |
| Journal | Plant cell reports (Plant Cell Rep) Vol. 29 Issue 3 Pg. 273-83 (Mar 2010) ISSN: 1432-203X [Electronic] Germany |
| PMID | 20094885 (Publication Type: Journal Article) |
| Chemical References |
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