Abstract | BACKGROUND:
Gastritis, an inflammation of gastric mucosa, may be due to many pathological factors and infection, such as with Helicobacter pylori. The use of experimental models of gastritis is important to evaluate the biochemical changes and study chemotherapeutic intervention. In a previous study we demonstrated an acute gastritis model induced by iodoacetamide. AIMS: Our objective in this study was to evaluate a new gastritis model induced by H. pylori infection in experimental rats in terms of certain biomarkers in serum and mucosal tissues in addition to histopathological examination. METHODS: RESULTS: Serum gastrin, IL-6, mucosal MPO activity, and PGE(2) demonstrated significant increases joined with a decreased serum pepsinogen I activity (P < 0.001). Immunohistochemistry demonstrated positive reaction for iNOS, nitrotyrosine and DNA fragmentation. CONCLUSIONS: Helicobacter pylori-induced gastritis models demonstrated massive oxidative stress and pronounced injury in mucosal tissue. Since our model in rats reflected the clinical picture of H. pylori infection, it can be considered as a consistent model to study chemotherapeutic intervention for this type of gastritis.
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Authors | Mohamed M Elseweidy, Mona M Taha, Nahla N Younis, Khadiga S Ibrahim, Hamdi A Hamouda, Mohamed A Eldosouky, Hala Soliman |
Journal | Digestive diseases and sciences
(Dig Dis Sci)
Vol. 55
Issue 10
Pg. 2770-7
(Oct 2010)
ISSN: 1573-2568 [Electronic] United States |
PMID | 20094782
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers
- Gastrins
- Interleukin-6
- Pepsinogen A
- Peroxidase
- Nitric Oxide Synthase Type II
- Nos2 protein, rat
- Dinoprostone
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Topics |
- Animals
- Biomarkers
(blood)
- Biopsy
- DNA Fragmentation
- Dinoprostone
(metabolism)
- Disease Models, Animal
- Female
- Gastric Mucosa
(metabolism, microbiology, pathology)
- Gastrins
(metabolism)
- Gastritis
(metabolism, microbiology, pathology)
- Helicobacter Infections
(metabolism, pathology)
- Helicobacter pylori
(isolation & purification)
- Humans
- Interleukin-6
(metabolism)
- Male
- Middle Aged
- Nitric Oxide Synthase Type II
(metabolism)
- Pepsinogen A
(metabolism)
- Peroxidase
(metabolism)
- Pyloric Antrum
(metabolism, microbiology, pathology)
- Rats
- Rats, Wistar
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