Abstract | BACKGROUND: OBJECTIVE: To investigate tacrolimus-dependent immunomodulation on gene expression alterations in human antigen-presenting cells which are stimulated with small-molecular-weight contact allergens. METHODS: Monocyte-derived dendritic cells (moDC) and THP-1 cells were stimulated with the contact sensitizer cinnamic aldehyde (CAld) and compared with the very strong experimental sensitizer 2,4,6-trinitrobenzene sulfonic acid (TNBS) in vitro. Quantitative PCR analysis was used to detect gene expression changes, particularly of interleukin (IL) 8, as an indicator of differential dendritic cell (DC) gene expression after sensitizer stimulation in the absence or presence of tacrolimus and betamethasone at two different concentrations. RESULTS: DC activation was clearly demonstrated by a significant IL-8 upregulation after 24 h, whereas tacrolimus or betamethasone alone did not affect IL-8 baseline expression. Betamethasone and, to a lesser extent, tacrolimus led to a marked reduction of chemical-induced IL-8 expression by TNBS and CAld. CONCLUSION:
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Authors | H Ott, J M Baron, R Heise, C Skazik, H F Merk |
Journal | Skin pharmacology and physiology
(Skin Pharmacol Physiol)
Vol. 23
Issue 1
Pg. 53-9
( 2010)
ISSN: 1660-5535 [Electronic] Switzerland |
PMID | 20090409
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | 2010 S. Karger AG, Basel. |
Chemical References |
- Immunosuppressive Agents
- Interleukin-8
- Acrolein
- Trinitrobenzenesulfonic Acid
- Betamethasone
- cinnamaldehyde
- Tacrolimus
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Topics |
- Acrolein
(analogs & derivatives, toxicity)
- Antigen-Presenting Cells
(drug effects, immunology)
- Betamethasone
(pharmacology)
- Cell Line, Tumor
- Dendritic Cells
(drug effects, immunology)
- Dermatitis, Allergic Contact
(immunology)
- Gene Expression Regulation
(drug effects)
- Humans
- Immunosuppressive Agents
(pharmacology)
- Interleukin-8
(genetics, immunology)
- Monocytes
(metabolism)
- Polymerase Chain Reaction
- Tacrolimus
(pharmacology)
- Trinitrobenzenesulfonic Acid
(toxicity)
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