HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Endotoxin recognition in fish results in inflammatory cytokine secretion not gene expression.

Abstract
Macrophages are phagocytes that have a central role in the organization of the immune system after an infection. These cells can recognize specific molecular components of micro-organisms (pathogen-associated molecular patterns, PAMPs) via specific receptors (PRRs) and elicit specific cellular responses. In the past, the expression of immune genes in response to different PAMPs has been characterized in different fish species. However, little is known about actual cytokine release. We characterized the secretion of tumour necrosis factor (TNF)-α in primary macrophage cultures of rainbow trout (Oncorhynchus mykiss) in response to several PAMPs by Western blot and compared this to the induction of TNF-α gene expression as well as other pro-inflammatory cytokines such as interleukin (IL)-1β and IL-6 and anti-viral molecules such as INF-α and Mx protein (Mx). We show that lipopolysaccharide (LPS) and zymosan are major inducers of TNF-α secretion, which is not initially linked to the induction of TNF-α mRNA expression. The secretion of TNF-α, but intriguingly not the expression, is also stimulated by ultrapure LPS meaning that, in fish, contaminants of commercial LPS preparations are better inducers of the inflammatory response. Moreover, we have characterized the signaling pathways that are activated by different PAMPs and the link between those pathways and the final step of TNF-α secretion: TNF-α shedding by TNF-α converting enzyme (TACE/ ADAM17). For the first time, we show that, in fish macrophages, TNF-α is processed by TACE-like activity and this cleavage is dependent upon the activation of ERK, p38MAPK and JNK signaling pathways by LPS.
AuthorsNerea Roher, Agnes Callol, Josep V Planas, Frederick W Goetz, Simon A MacKenzie
JournalInnate immunity (Innate Immun) Vol. 17 Issue 1 Pg. 16-28 (Feb 2011) ISSN: 1753-4267 [Electronic] United States
PMID20083499 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Endotoxins
  • Interleukin-1beta
  • Interleukin-6
  • Lipopolysaccharides
  • Protein Kinase Inhibitors
  • Tumor Necrosis Factor-alpha
  • Dactinomycin
  • Zymosan
  • Cycloheximide
  • Extracellular Signal-Regulated MAP Kinases
  • JNK Mitogen-Activated Protein Kinases
  • p38 Mitogen-Activated Protein Kinases
  • ADAM Proteins
  • tumor necrosis factor-alpha convertase
Topics
  • ADAM Proteins (antagonists & inhibitors, metabolism)
  • Animals
  • Cells, Cultured
  • Cycloheximide (pharmacology)
  • Dactinomycin (pharmacology)
  • Endotoxins (pharmacology)
  • Extracellular Signal-Regulated MAP Kinases (antagonists & inhibitors, metabolism)
  • Gene Expression (drug effects, genetics)
  • Interleukin-1beta (genetics)
  • Interleukin-6 (genetics)
  • JNK Mitogen-Activated Protein Kinases (antagonists & inhibitors, metabolism)
  • Kinetics
  • Lipopolysaccharides (pharmacology)
  • Macrophages (drug effects, metabolism, secretion)
  • Oncorhynchus mykiss
  • Protein Kinase Inhibitors (pharmacology)
  • Signal Transduction (drug effects, physiology)
  • Tumor Necrosis Factor-alpha (genetics, secretion)
  • Zymosan (pharmacology)
  • p38 Mitogen-Activated Protein Kinases (antagonists & inhibitors, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: