Abstract | OBJECTIVE: DESIGN: Prospective, case-control genetic association study. SETTING: Three tertiary children's hospitals and one adult healthcare system. PATIENTS: A total of 800 pediatric patients and 393 adult patients. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: Genetic variation in the myosin light chain kinase gene was examined. The pediatric cohort was predominantly composed of African American (n = 443) and Caucasian (n = 253) children. A total of 393 patients made up the adult cohort. Within the pediatric cohort, single nucleotide polymorphisms rs16834493, rs820463, and rs9840993 were genotyped in the African American patients, whereas single nucleotide polymorphisms rs960224, rs33264, rs11718105, and rs9289225 were genotyped in the Caucasian patients. One single nucleotide polymorphism (rs820336) was genotyped in both groups. Genotyping in the adult cohort included rs820336, rs860224, rs33264, and rs11718105. Genotyping was performed using the Taqman Assay. Data were analyzed separately for African Americans and Caucasians and for children and adults. No associations were observed between the myosin light chain kinase gene single nucleotide polymorphisms genotyped in children with community-acquired pneumonia and increased severity of lung injury. Similarly, no associations were observed between myosin light chain kinase gene single nucleotide polymorphisms genotyped in adults with community-acquired pneumonia and increased severity of lung injury. CONCLUSIONS:
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Authors | Rebecca Russell, Michael W Quasney, Nadine Halligan, Shun-Hwa Li, Pippa Simpson, Grant Waterer, Richard G Wunderink, Mary K Dahmer |
Journal | Pediatric critical care medicine : a journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies
(Pediatr Crit Care Med)
Vol. 11
Issue 6
Pg. 731-6
(Nov 2010)
ISSN: 1529-7535 [Print] United States |
PMID | 20081554
(Publication Type: Comment, Journal Article, Multicenter Study, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Myosin-Light-Chain Kinase
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Topics |
- Adolescent
- Adult
- Case-Control Studies
- Child
- Child, Preschool
- Female
- Genetic Predisposition to Disease
- Genetic Variation
- Genotype
- Haplotypes
- Humans
- Infant
- Infant, Newborn
- Lung Injury
(enzymology, ethnology, genetics)
- Male
- Myosin-Light-Chain Kinase
(genetics)
- Pneumonia
(enzymology, ethnology, genetics)
- Polymorphism, Single Nucleotide
- Positive-Pressure Respiration
- Prospective Studies
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