Detection of Notch signaling molecules in cemento-ossifying fibroma of the jaws.

Abstract

BACKGROUND:

The aim of this study was to evaluate the roles of Notch signaling in the oncogenesis and cytodifferentiation of cemento-ossifying fibroma, the expressions of Notch receptors and ligands were detected in COF and normal jaw bones.

MATERIALS AND METHODS:

The expressions of Notch1, Notch3, Jagged1, and Jagged2 were detected by reverse transcriptase polymerase chain reaction and immunohistochemistry respectively in 16 cases of normal bone tissues and 12 cases of COF of the jaws.

RESULTS:

The mRNAs expressions of Notch1, Notch3, Jagged1, and Jagged2 were detected in all specimens. The expression levels of mRNAs in COF were higher than those in normal bones. In COF, Notch proteins staining were showed extensively distribution in fibroblasts and osteoblasts. In normal bone tissue, Notch proteins were expressed in osteoblasts, whereas proteins staining were weaker than those in COF, but no detection in fibroblast-like bone marrow stroma cells. The expressions of Notch receptors and ligands were not detected in cementum-like products or bone matrices.

CONCLUSION:

Our data suggest that Notch signaling may participate in controlling cell differentiation and proliferation in normal bone and COF of the jaws. Notch signaling disorder may be a molecular incident in COF occurrence and development.

• Authors: Tong-Han Zhang, Hai-Chao Liu, Gui-Qing Liao, Yu-Jie Liang, Mei Chu, Chang-Qing Wan, Li-Zhong Liang, Guang-Sen Zheng
• Journal: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology (J Oral Pathol Med) Vol. 39 Issue 3 Pg. 263-8 (Mar 2010) ISSN: 1600-0714 [Electronic] Denmark
• PMID: 20040020 (Publication Type: Journal Article)

Chemical References

• Calcium-Binding Proteins
• Intercellular Signaling Peptides and Proteins
• JAG1 protein, human
• JAG2 protein, human
• Jagged-1 Protein
• Jagged-2 Protein
• Membrane Proteins
• NOTCH1 protein, human
• NOTCH3 protein, human
• Receptor, Notch1
• Receptor, Notch3
• Receptors, Notch
• Serrate-Jagged Proteins

Topics

• Bone Marrow Cells (pathology)
• Bone Matrix (pathology)
• Calcium-Binding Proteins (analysis)
• Cell Differentiation
• Cell Proliferation
• Cell Transformation, Neoplastic (pathology)
• Dental Cementum (pathology)
• Fibroblasts (pathology)
• Humans
• Intercellular Signaling Peptides and Proteins (analysis)
• Jagged-1 Protein
• Jagged-2 Protein
• Jaw (pathology)
• Jaw Neoplasms (pathology)
• Membrane Proteins (analysis)
• Odontogenic Tumors (pathology)
• Osteoblasts (pathology)
• Receptor, Notch1 (analysis)
• Receptor, Notch3
• Receptors, Notch (analysis)
• Serrate-Jagged Proteins
• Stromal Cells (pathology)