Abstract | OBJECTIVE: DESIGN: Prospective, randomized, controlled experimental study. SETTING: Animal laboratory, university hospital. SUBJECTS: Male Sprague-Dawley rats. INTERVENTIONS: A total of 40 rats were immobilized in one hind limb by pinning knee and ankle joints. Rats received either continuous pyridostigmine (15 mg.kg.day) or saline subcutaneously via implanted osmotic pumps. MEASUREMENTS AND MAIN RESULTS: After 7 days and 14 days of immobilization, neuromuscular function, atracurium pharmacodynamics, and expression of acetylcholine receptors were evaluated. At 7 days and 14 days after immobilization, muscle force decreased in all untreated groups, whereas effective doses for paralysis with atracurium and acetylcholine receptor number in the tibialis were significantly increased. Pyridostigmine-treated rats showed a significantly improved muscle force and muscle mass in the immobilized limb. This was associated with an attenuation of acetylcholine receptor up-regulation in the respective leg. At this time, the dose-response curve for atracurium on the immobilized side was shifted to the left in the pyridostigmine group. After 14 days, muscle tension was still less depressed with pyridostigmine infusion, and resistance to the effects of atracurium was still attenuated. However, there were no differences in acetylcholine receptor expression between the immobilized sides of both groups. CONCLUSIONS:
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Authors | Christiane G Frick, Marc Helming, J A Jeevendra Martyn, Manfred Blobner, Heidrun Fink |
Journal | Critical care medicine
(Crit Care Med)
Vol. 38
Issue 3
Pg. 922-7
(Mar 2010)
ISSN: 1530-0293 [Electronic] United States |
PMID | 20009758
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Cholinesterase Inhibitors
- Neuromuscular Nondepolarizing Agents
- Receptors, Cholinergic
- Atracurium
- Pyridostigmine Bromide
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Topics |
- Animals
- Atracurium
(pharmacology)
- Cholinesterase Inhibitors
(pharmacology)
- Disease Models, Animal
- Hindlimb
(innervation)
- Immobilization
- Infusion Pumps
- Infusions, Subcutaneous
- Male
- Muscle Contraction
(drug effects, physiology)
- Muscle Strength
(drug effects, physiology)
- Muscle Weakness
(physiopathology)
- Muscular Atrophy
(physiopathology)
- Neuromuscular Junction
(drug effects, physiopathology)
- Neuromuscular Nondepolarizing Agents
(pharmacology)
- Pyridostigmine Bromide
(pharmacology)
- Rats
- Rats, Sprague-Dawley
- Receptors, Cholinergic
(drug effects, physiology)
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