The influence of common gene variants of the xenobiotic receptor (PXR) in genetic susceptibility to intrahepatic cholestasis of pregnancy.

Abstract

BACKGROUND:

The xenobiotic nuclear pregnane X receptor is implicated in many physiological pathways and diseases, including bile acid detoxification and cholestasis. Aim To estimate the contribution of common gene variants of the xenobiotic receptor (pregnane X receptor, PXR) to genetic susceptibility to intrahepatic cholestasis of pregnancy.

METHODS:

A total of 101 intrahepatic cholestasis of pregnancy patients and 171 healthy pregnant women in the third trimester of their pregnancies were included. Four tag single nucleotide polymorphisms (SNPs) (rs12488820 C/T, rs2472671 C/T, rs2461823 A/G, and rs1054191 A/G) encompassing 36 kb in chromosome 3, with a minor allele frequency > or =0.10 and representing 33 polymorphic sites were genotyped. Besides these, three additional SNPs (rs3814057, rs6785049, and rs7643645) were included because they showed previous evidence of functionality.

RESULTS:

Genotypic test for single SNPs showed that rs2461823 genotypes were significantly associated with intrahepatic cholestasis of pregnancy (P < 0.0069), OR per G allele: 1.44, 95% CI: 1.01-2.05, P < 0.042. The Cochran-Armitage test for trend and the allelic test showed a significant association with disease status (P < 0.04 and 0.03 respectively), G being the risk allele. A positive association between rs2461823 and ALT, AST, and bilirubin concentrations was observed. Neonate birth weight adjusted by the Capurro index was significantly associated with rs2461823 (P < 0.05); the proportion of the total variation attributed to rs2461823 genotypes was 7.8%.

CONCLUSION:

Common PXR polymorphisms may contribute to the genetic susceptibility to intrahepatic cholestasis of pregnancy.

• Authors: G Castaño, A Burgueño, T Fernández Gianotti, C J Pirola, S Sookoian
• Journal: Alimentary pharmacology & therapeutics (Aliment Pharmacol Ther) Vol. 31 Issue 5 Pg. 583-92 (Mar 2010) ISSN: 1365-2036 [Electronic] England
• PMID: 19958310 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bile Acids and Salts
• Pregnane X Receptor
• Receptors, Steroid
• Xenobiotics

Topics

• Adult
• Analysis of Variance
• Bile Acids and Salts (metabolism)
• Birth Weight
• Cholestasis, Intrahepatic (epidemiology, genetics, metabolism)
• Chromosomes, Human, Pair 3 (genetics)
• Female
• Gene Frequency (genetics)
• Genetic Association Studies
• Genetic Predisposition to Disease (epidemiology)
• Genetic Variation
• Gestational Age
• Homeostasis
• Humans
• Incidence
• Infant, Newborn
• Polymorphism, Single Nucleotide (genetics)
• Pregnancy
• Pregnancy Complications (epidemiology, genetics, metabolism)
• Pregnancy Trimester, Third
• Pregnane X Receptor
• Receptors, Steroid (genetics)
• Seasons
• South America (epidemiology)
• Xenobiotics