Abstract |
Two fast methods for the purification of mouse monoclonal antibodies from ascites, fluids using Blue- DEAE and 'thiophilic' adsorbent (T gel) in the FPLC system are described. Blue- DEAE chromatography is useful only for IgG1, IgG2a and IgG2b monoclonal antibody purification. T gel is a satisfactory adsorbent for IgG2b purification. Other IgG subclasses and IgM can also be obtained by simple one-step procedures, but the preparations contain small amount of high molecular weight contaminants.
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Authors | E Juronen, J Parik, P Toomik |
Journal | Journal of immunological methods
(J Immunol Methods)
Vol. 136
Issue 1
Pg. 103-9
(Jan 24 1991)
ISSN: 0022-1759 [Print] Netherlands |
PMID | 1995702
(Publication Type: Journal Article)
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Chemical References |
- Antibodies, Monoclonal
- Ethanolamines
- Gels
- Immunoglobulin G
- 2-diethylaminoethanol
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Topics |
- Animals
- Antibodies, Monoclonal
(isolation & purification)
- Ascitic Fluid
(immunology)
- Chromatography, Liquid
- Ethanolamines
- Gels
- Immunoglobulin G
(isolation & purification)
- Mice
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