Delivery of rifampicin-PLGA microspheres into alveolar macrophages is promising for treatment of tuberculosis.

Abstract

Inhalation delivery of poly(lactic-co-glycolic) acid (PLGA) microspheres (MS) loaded with the anti-tuberculosis agent rifampicin (RFP-PLGA MS) to alveolar macrophage (M phi) cells could be an effective drug delivery system for the treatment of tuberculosis. To examine this possibility, we studied (1) the bactericidal effect of RFP-PLGA MS on Mycobacterium bovis Bacillus Calmette-Guérin (BCG)-infected rat alveolar M phi NR8383 cells, and (2) changes in the biochemical events induced in these cells by the uptake of RFP-PLGA MS. The amount of intracellular RFP imported into the M phi s by RFP-PLGA MS containing 0.25 and 2.50 microg RFP/mL was more than twice and ten times, respectively, than that attained with 5.00 microg/mL of RFP solution; and the MS exerted more potent bactericidal effect on BCG inside M phi cells than 5.00 microg RFP/mL solution after incubation for 7 days. RFP-PLGA MS little affected the viability of M phi cells, whereas the polystyrene latex (PSL) MS used as a reference decreased it significantly. RFP-PLGA MS did not stimulate the production of tumor necrosis factor-alpha (TNF-alpha), nitric oxide, interleukin-10 (IL-10), and transforming growth factor-beta1 (TGF-beta1) by the M phi cells, whereas PSL MS stimulated all of these mediators except IL-10. We conclude that RFP-PLGA MS are bio-safe microspheres due to their "silent" nature when taken into M phi cells and that they are promising for the treatment of tuberculosis by pulmonary inhalation.

• Authors: Keiji Hirota, Taizo Hasegawa, Takehisa Nakajima, Hiroyuki Inagawa, Chie Kohchi, Gen-Ichiro Soma, Kimiko Makino, Hiroshi Terada
• Journal: Journal of controlled release : official journal of the Controlled Release Society (J Control Release) Vol. 142 Issue 3 Pg. 339-46 (Mar 19 2010) ISSN: 1873-4995 [Electronic] Netherlands
• PMID: 19951729 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: (c) 2009 Elsevier B.V. All rights reserved.

Chemical References

• Antibiotics, Antitubercular
• Drug Carriers
• Tumor Necrosis Factor-alpha
• Interleukin-10
• Polylactic Acid-Polyglycolic Acid Copolymer
• Polyglycolic Acid
• Nitric Oxide
• Lactic Acid
• Rifampin

Topics

• Animals
• Antibiotics, Antitubercular (administration & dosage, pharmacokinetics, therapeutic use)
• Cell Line
• Cell Survival (drug effects)
• Drug Carriers (chemistry)
• Interleukin-10 (biosynthesis, immunology)
• Lactic Acid (chemistry)
• Macrophages, Alveolar (immunology, metabolism, microbiology)
• Microspheres
• Mycobacterium bovis (drug effects)
• Nitric Oxide (biosynthesis, immunology)
• Phagocytosis (drug effects, immunology)
• Polyglycolic Acid (chemistry)
• Polylactic Acid-Polyglycolic Acid Copolymer
• Rats
• Rifampin (administration & dosage, pharmacokinetics, therapeutic use)
• Tissue Distribution
• Tuberculosis (drug therapy)
• Tumor Necrosis Factor-alpha (biosynthesis, immunology)