The aim of this study was to identify a phenolic
prodrug compound that is minimally metabolized by rat liver microsomes, but yet could form
quinone reactive intermediates in
melanoma cells as a result of its bioactivation by
tyrosinase. In current work, we investigated 24 phenolic compounds for their metabolism by
tyrosinase, rat liver microsomes and their toxicity towards murine B16-F0 and human SK-MEL-28
melanoma cells. A linear correlation was found between toxicities of phenolic analogs towards SK-MEL-28 and B16-F0
melanoma cells, suggesting similar mechanisms of toxicity in both cell lines. 4-HEB was identified as the lead compound. 4-HEB (IC(50) 48h, 75muM) showed selective toxicity towards five melanocytic
melanoma cell lines SK-MEL-28, SK-MEL-5, MeWo, B16-F0 and B16-F10, which express functional
tyrosinase, compared to four non-
melanoma cells lines SW-620, Saos-2, PC3 and BJ cells and two amelanotic SK-MEL-24, C32 cells, which do not express functional
tyrosinase. 4-HEB caused significant intracellular GSH depletion, ROS formation, and showed significantly less toxicity to
tyrosinase specific
shRNA transfected SK-MEL-28 cells. Our findings suggest that presence of a phenolic group in 4-HEB is critical for its selective toxicity towards
melanoma cells.