Influence of baicalin on TNF-alpha mRNA, caspase-3 and P-selectin expression in pancreatic tissue of rats with severe acute pancreatitis.

Abstract

OBJECTIVE:

Baicalin reduces the severity of severe acute pancreatitis (SAP) in a rat model. This study was carried out to examine the effect of baicalin on TNF-alpha mRNA, caspase-3 and P-selectin protein expression in the pancreas of rats with SAP.

METHODS:

Rats with SAP were randomly assigned to untreated, baicalin-treated, octreotide-treated and sham-operated group. The TNF-alpha mRNA expression level, caspase-3 and P-selectin protein expression levels of pancreatic tissue were measured 3, 6 and 12 hours after operation.

RESULTS:

The caspase-3 protein expression level was significantly higher in the treated groups than that in the untreated group at 3 hours (p <0.01). P-selectin protein expression level in pancreas of the treated groups was significantly lower than that of the untreated group (p <0.05 or <0.01). TNF-alpha mRNA expression level in pancreas decreased in baicalintreated group while untreated group increased at different time points (p <0.05).

CONCLUSION:

Baicalin increases caspase-3 expression and inhibits TNF-alpha mRNA and P-selectin protein expression in pancreatic tissue of SAP rats.

• Authors: Zhang Xiping, Tian Hua, Zhang Jie, Chen Li, Cai Yang, Lu Bei, Yang Qijun, Ye Jing, Yuan Wengqin, Zhou Ninnin
• Journal: Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology (Indian J Gastroenterol) 2009 Jul-Aug Vol. 28 Issue 4 Pg. 131-5 ISSN: 0975-0711 [Electronic] India
• PMID: 19937174 (Publication Type: Journal Article)

Chemical References

• Enzyme Inhibitors
• Flavonoids
• P-Selectin
• Tumor Necrosis Factor-alpha
• baicalin
• Caspase 3

Topics

• Animals
• Caspase 3 (drug effects)
• Disease Models, Animal
• Enzyme Inhibitors (pharmacology)
• Flavonoids (pharmacology)
• Gene Expression Regulation, Neoplastic (drug effects)
• Male
• P-Selectin (drug effects)
• Pancreatitis (drug therapy, genetics)
• Rats
• Rats, Sprague-Dawley
• Severity of Illness Index
• Tumor Necrosis Factor-alpha (drug effects)