A vaccine for hypertension based on peptide AngI-R: a pilot study.

Abstract

AIMS:

We aim to modulate the renin-angiotensin system (RAS) by active immunization against angiotensin I/II, potentially by a novel type of peptide--'AngI-R' for the treatment of hypertension.

METHOD:

27 spontaneous hypertensive rats (SHRs) were randomly divided into 3 groups (n=9). Effect of AngI-R on systolic blood pressure of SHRs was determined by its subcutaneous injection into SHRs with anti-angiotensin peptide AngI-R, captopril, physiological salt solution in the same schedule. Systolic blood pressure was monitored by tail-cuff arterial blood pressure measurement method and then calculated from the average of three measurements in each animal at a desired time (0, 4, 8, 16W). AngI, AngII and anti-AngI antibody were detected by ELISA and western blotting method. At the end, the pathologic changes in different organs of SHRs were observed.

RESULT:

The results exhibited a marked effect to lower the systolic blood pressure (-15 mmHg) of SHRs after subcutaneous injection with peptide AngI-R. The anti-hypertensive effect lasted for over 1 month. The level of AngI and AngII in the vaccinated group, after administration of AngI-R was lower than the one in the control group and the pre-administered group. The anti-AngI/II antibody titre in the experiment group significantly increased.

CONCLUSION:

The peptide AngI-R was able to decrease the blood pressure of SHRs. The peptide AngI-R induced an immune response to inhibit AngI and AngII, resulting in the decrease in blood pressure in SHRs.

• Authors: Fang Hong, Wu Yong Quan, Rabindra Pandey, Shen Yi, Liu Chi, Li Zhan Xia, Ma Yuan, Lou Ming
• Journal: International journal of cardiology (Int J Cardiol) Vol. 148 Issue 1 Pg. 76-84 (Apr 01 2011) ISSN: 1874-1754 [Electronic] Netherlands
• PMID: 19932924 (Publication Type: Comparative Study, Journal Article)
• Copyright: Copyright © 2009 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Isoantibodies
• Vaccines, Subunit
• Angiotensin II
• Angiotensin I

Topics

• Angiotensin I (administration & dosage, antagonists & inhibitors, therapeutic use)
• Angiotensin II (administration & dosage, antagonists & inhibitors, therapeutic use)
• Animals
• Blood Pressure (drug effects, physiology)
• Hypertension (pathology, prevention & control)
• Isoantibodies (biosynthesis)
• Male
• Pilot Projects
• Rabbits
• Random Allocation
• Rats
• Rats, Inbred SHR
• Vaccines, Subunit (administration & dosage, therapeutic use)