Abstract | OBJECTIVE: Primary mediastinal B-cell lymphoma (PMBCL) and Hodgkin lymphoma (HL) share many biological and clinical characteristics supporting a common pathogenetic pathway. Interleukin (IL)-13 has an important pathophysiological role in HL. In this study, we asked the question of whether IL-13 is a major contributor to the observed difference in features of inflammation between HL and PMBCL. MATERIALS AND METHODS: RESULTS: Stimulation of the PMBCL cell line Karpas-1106P with IL-13 or IL-4 induced a proinflammatory phenotype similar to that of the HL cell line L1236. Upon interleukin stimulation of the PMBCL cell line, the cellular size increased and cells became multinucleated. Cells also expressed CysLT1R and 15-LO-1, and produced the proinflammatory eoxins. The IL-13 or IL-4 treated PMBCL cell line and the HL cell line secreted a similar set of cytokines such as IL-6, tumor necrosis factor-alpha, interferon-inducible protein-10, interferon-gamma, and RANTES. CONCLUSIONS:
IL-13 or IL-4 stimulation of the PMBCL cell line Karpas-1106P induced an inflammatory phenotype that resembles that of the HL cell line. Our results suggest that the autocrine release of IL-13 in HL is one critical factor that can at least partly explain the difference in phenotype between these two lymphoma entities.
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Authors | Erik Andersson, Frida Schain, Jan Sjöberg, Magnus Björkholm, Hans-Erik Claesson |
Journal | Experimental hematology
(Exp Hematol)
Vol. 38
Issue 2
Pg. 116-23
(Feb 2010)
ISSN: 1873-2399 [Electronic] Netherlands |
PMID | 19931589
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2010 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Cytokines
- Interleukin-13
- Leukotrienes
- Receptors, Leukotriene
- Interleukin-4
- Arachidonate 15-Lipoxygenase
- leukotriene D4 receptor
- Calcium
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Topics |
- Arachidonate 15-Lipoxygenase
(metabolism)
- Calcium
(metabolism)
- Cell Line, Tumor
- Cell Nucleus
(ultrastructure)
- Cell Size
- Cytokines
(metabolism)
- Hodgkin Disease
(pathology, physiopathology)
- Humans
- Inflammation
(pathology)
- Interleukin-13
(pharmacology)
- Interleukin-4
(pharmacology)
- Leukotrienes
(biosynthesis)
- Lymphoma, B-Cell
(pathology, physiopathology, ultrastructure)
- Phenotype
- Receptors, Leukotriene
(physiology)
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