IL-9 is a
cytokine of great current interest associated with allergic/Th2 responses. High levels of
IL-9 are present in bronchial secretions from infants with respiratory syncytial virus (RSV)
bronchiolitis. To test its effects in RSV disease with a Th2 profile, BALB/c mice were vaccinated with recombinant vaccinia virus expressing the RSV
G protein. On RSV challenge, immunized mice developed augmented disease characterized by enhanced pulmonary Th2 and local
IL-9 production peaking on days 7-10 of
RSV infection. Depletion with anti-IL-9 Ab at vaccination or RSV challenge enhanced viral clearance. Depletion only at challenge had no effect on disease severity, whereas depletion at immunization and challenge enhanced Th1 responses, inhibited virus-specific
IgG1 production, and enhanced disease severity. By contrast, depletion of
IL-9 at immunization boosted
IgG2a and inhibited the Th2 response and disease during subsequent
infection without a concomitant increase in type 1
cytokines. Adoptive transfer of secondary memory CD4 T cells from the spleens of IL-9-depleted mice into naive recipients replicated many of the effects of depletion, indicating that
IL-9 acts via CD4 T cells. Therefore,
IL-9 is a previously unknown but key modulator of
antiviral immunity, regulating T and B cell responses and having potent and specific effects on viral
lung disease.