Abstract | PURPOSE: MATERIALS AND METHODS:
Cystitis in WT C57BL6 mice was induced with intraperitoneal cyclophosphamide. Retrieved bladders were analyzed using histology, immunohistochemistry, reverse transcriptase-polymerase chain reaction and Western blot for glycosylation associated factors. RESULTS: CONCLUSIONS: To our knowledge we report the first study of alterations in O-GlcNAc mediated glycosylation activity in bladders with cyclophosphamide induced cystitis. Glycosylation may have a significant role in the bladder wound healing process. Future studies of the glycosylation signaling pathways in the bladder would assist in future potential therapy for bladder inflammatory disease and cancer by elucidating pathways that guide bladder development and wound healing.
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Authors | Seyung Chung, Dae-Ook Kang, Jonathan Yamzon, David Warburton, Chester J Koh |
Journal | The Journal of urology
(J Urol)
Vol. 183
Issue 1
Pg. 351-6
(Jan 2010)
ISSN: 1527-3792 [Electronic] United States |
PMID | 19914650
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cyclophosphamide
- N-Acetylglucosaminyltransferases
- UDP-N-acetylglucosamine-peptide beta-N-acetylglucosaminyltransferase
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Topics |
- Animals
- Cyclophosphamide
(administration & dosage)
- Cystitis
(chemically induced, enzymology)
- Down-Regulation
(physiology)
- Glycosylation
- Mice
- N-Acetylglucosaminyltransferases
(physiology)
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